Kokjohn T A, Miller R V
J Bacteriol. 1985 Aug;163(2):568-72. doi: 10.1128/jb.163.2.568-572.1985.
The recA gene of Pseudomonas aeruginosa PAO has been isolated and introduced into Escherichia coli K-12. Resistance to killing by UV irradiation was restored in several RecA-E. coli K-12 hosts by the P. aeruginosa gene, as was resistance to methyl methanesulfonate. Recombination proficiency was also restored, as measured by HfrH-mediated conjugation and by the ability to propagate Fec-phage lambda derivatives. The cloned P. aeruginosa recA gene restored both spontaneous and mitomycin C-stimulated induction of lambda prophage in lysogens of a recA strain of E. coli K-12.
铜绿假单胞菌PAO的recA基因已被分离出来并导入大肠杆菌K-12。铜绿假单胞菌基因在几个RecA - 大肠杆菌K-12宿主中恢复了对紫外线照射杀伤的抗性,对甲磺酸甲酯的抗性也得以恢复。通过HfrH介导的接合以及繁殖Fec - 噬菌体λ衍生物的能力来衡量,重组能力也得到了恢复。克隆的铜绿假单胞菌recA基因在大肠杆菌K-12的recA菌株的溶原菌中恢复了λ原噬菌体的自发诱导和丝裂霉素C刺激诱导。