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2
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Expression of the recA gene of Pseudomonas aeruginosa PAO is inducible by DNA-damaging agents.铜绿假单胞菌PAO的recA基因表达可被DNA损伤剂诱导。
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Conservation of an ATP-binding domain among RecA proteins from Proteus vulgaris, Erwinia carotovora, Shigella flexneri, and Escherichia coli K-12 and B/r.普通变形杆菌、胡萝卜软腐欧文氏菌、福氏志贺菌以及大肠杆菌K-12和B/r的RecA蛋白中ATP结合结构域的保守性。
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本文引用的文献

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Segregation of New Lysogenic Types during Growth of a Doubly Lysogenic Strain Derived from Escherichia Coli K12.源于大肠杆菌K12的双重溶源菌株生长过程中新溶源类型的分离
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ISOLATION AND CHARACTERIZATION OF RECOMBINATION-DEFICIENT MUTANTS OF ESCHERICHIA COLI K12.大肠杆菌K12重组缺陷突变体的分离与鉴定
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Interspecies recA protein substitution in Escherichia coli and Proteus mirabilis.大肠杆菌和奇异变形杆菌中的种间RecA蛋白替代
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Overproduction of the Escherichia coli recA protein without stimulation of its proteolytic activity.大肠杆菌recA蛋白过度产生而未刺激其蛋白水解活性。
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Recognition of duplex DNA containing single-stranded regions by recA protein.RecA蛋白对含有单链区域的双链DNA的识别。
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Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli.大肠杆菌中的诱变作用及对脱氧核糖核酸损伤的诱导反应
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Linkage map of Escherichia coli K-12, edition 7.大肠杆菌K-12连锁图谱,第7版。
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Isolation and characterization of the recA gene of Rhizobium meliloti.苜蓿根瘤菌recA基因的分离与鉴定
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Autodigestion of lexA and phage lambda repressors.LexA和噬菌体λ阻遏物的自催化作用。
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铜绿假单胞菌PAO的recA基因的分子克隆与特性分析

Molecular cloning and characterization of the recA gene of Pseudomonas aeruginosa PAO.

作者信息

Kokjohn T A, Miller R V

出版信息

J Bacteriol. 1985 Aug;163(2):568-72. doi: 10.1128/jb.163.2.568-572.1985.

DOI:10.1128/jb.163.2.568-572.1985
PMID:2991195
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC219159/
Abstract

The recA gene of Pseudomonas aeruginosa PAO has been isolated and introduced into Escherichia coli K-12. Resistance to killing by UV irradiation was restored in several RecA-E. coli K-12 hosts by the P. aeruginosa gene, as was resistance to methyl methanesulfonate. Recombination proficiency was also restored, as measured by HfrH-mediated conjugation and by the ability to propagate Fec-phage lambda derivatives. The cloned P. aeruginosa recA gene restored both spontaneous and mitomycin C-stimulated induction of lambda prophage in lysogens of a recA strain of E. coli K-12.

摘要

铜绿假单胞菌PAO的recA基因已被分离出来并导入大肠杆菌K-12。铜绿假单胞菌基因在几个RecA - 大肠杆菌K-12宿主中恢复了对紫外线照射杀伤的抗性,对甲磺酸甲酯的抗性也得以恢复。通过HfrH介导的接合以及繁殖Fec - 噬菌体λ衍生物的能力来衡量,重组能力也得到了恢复。克隆的铜绿假单胞菌recA基因在大肠杆菌K-12的recA菌株的溶原菌中恢复了λ原噬菌体的自发诱导和丝裂霉素C刺激诱导。