Instituto de Biología, Facultad de Ciencias Exactas y Naturales , Universidad de Antioquia , Calle 67 #53-108 , Medellín AA 1226 , Colombia.
Programa de Posgrado del Centro de la República (PROPAC), Research and Graduate Studies in Food Science, School of Chemistry , Universidad Autónoma de Querétaro , Santiago de Querétaro 76010 , México.
J Agric Food Chem. 2018 Jul 18;66(28):7358-7366. doi: 10.1021/acs.jafc.8b01604. Epub 2018 Jul 6.
Berry consumption is associated with colorectal-cancer chemoprevention, but digestive conditions can affect this property. The bioaccessibility and apparent permeability coefficients of bioactive compounds from Andean Berry Juice (ABJ) after in vitro gastrointestinal digestion and colonic fermentation were analyzed. The antiproliferative effect of the fermented nondigestible fraction was evaluated against SW480 colon-adenocarcinoma cells. Gallic acid displayed the highest bioaccessibility in the mouth, stomach, small intestine, and colon. However, chlorogenic acid exhibited the highest apparent permeability coefficients (up to 1.98 × 10 cm/s). The colonic-fermentation fraction showed an increase of ≥50% antiproliferative activity against SW480 cells (19.32%, v/v), equivalent to those of gallic acid (13.04 μg/g), chlorogenic acid (7.07 μg/g), caffeic acid (0.40 μg/g), ellagic acid (7.32 μg/g), rutin (6.50 μg/g), raffinose (0.14 mg/g), stachyose (0.70 mg/g), and xylose (9.41 mg/g). Bioactive compounds from ABJ are bioaccessible through the gastrointestinal tract and colon fermentation, resulting in antiproliferative activity.
浆果的摄入与结直肠癌的化学预防有关，但消化状况可能会影响这一特性。分析了安第斯浆果汁（ABJ）中生物活性化合物在体外胃肠道消化和结肠发酵后的生物利用度和表观渗透系数。评估了发酵不可消化部分对 SW480 结肠腺癌细胞的增殖抑制作用。没食子酸在口腔、胃、小肠和结肠中的生物利用度最高。然而，绿原酸表现出最高的表观渗透系数（高达 1.98×10cm/s）。结肠发酵部分对 SW480 细胞的增殖抑制活性增加了≥50%（19.32%，v/v），与没食子酸（13.04μg/g）、绿原酸（7.07μg/g）、咖啡酸（0.40μg/g）、鞣花酸（7.32μg/g）、芦丁（6.50μg/g）、棉子糖（0.14mg/g）、水苏糖（0.70mg/g）和木糖（9.41mg/g）相当。ABJ 中的生物活性化合物可通过胃肠道和结肠发酵被生物利用，从而具有增殖抑制活性。
