Biomedical Research and Innovation Group (GIB) - Instituto Tecnológico Metropolitano (ITM), Calle 54A #30-31, 050034 Medellin, Antioquia, Colombia.
Programa de Posgrado del Centro de la República (PROPAC), Research and Graduate Program in Food Science, School of Chemistry, Universidad Autónoma de Querétaro, Cerro de las Campanas S/N, 76010, Santiago de Querétaro, Qro., Mexico.
Food Res Int. 2020 Nov;137:109541. doi: 10.1016/j.foodres.2020.109541. Epub 2020 Jul 16.
V. meridionale Swartz is an underutilized Andean Berry that has been linked to several health benefits potentially derived from its anti-inflammatory effects. This research aimed to evaluate the impact of Andean Berry Juice (ABJ) combined with Aspirin in the modulation of anti-inflammatory markers from LPS-stimulated RAW 264.7 macrophages. The chemical characterization of ABJ showed a high content of polyphenols, mainly gallic acid (659-75 μg/g) and cyanidin chloride (418.61 μg/mL). Compared to LPS-stimulated macrophages, ABJ, Aspirin, and its combination reduced NO and ROS production (3.26-42.55 and 17.59-65.68%, respectively). In comparison, the half inhibitory concentration of NO reduction (IC) was found at 7.69% v/v (ABJ) and 24.48 mM (Aspirin). Compared to the pro-inflammatory control (LPS), ABJ reduced IL-1β, MCP-1, and GCSF; Aspirin decreased IL1R1, MCP-1, GMCSF, GCSF, and TNF-α; and the ABJ + Aspirin treatment reduced IL1R, GMCSF, and CXCL10. The in silico interaction of cytokines and the prediction of potential binding interactions suggested CCR1, CCR5, and NF-kB modulation. These results showed the anti-inflammatory potential of underutilized South American berries and their co-adjuvant effect with known drugs such as Aspirin in the resolution of inflammatory-derived conditions. This is the first report of the anti-inflammatory effects of V. meridionale Swartz juice in combination with Aspirin on LPS-challenged RAW 264.7 macrophages.
V. meridionale Swartz 是一种未充分利用的安第斯莓，其抗炎作用可能与其多种健康益处有关。本研究旨在评估安第斯莓汁（ABJ）与阿司匹林联合应用对 LPS 刺激的 RAW 264.7 巨噬细胞中抗炎标志物的调节作用。ABJ 的化学特征分析表明，其含有丰富的多酚类物质，主要为没食子酸（659-75μg/g）和氯化矢车菊素（418.61μg/mL）。与 LPS 刺激的巨噬细胞相比，ABJ、阿司匹林及其联合应用均能降低 NO 和 ROS 的产生（分别为 3.26-42.55%和 17.59-65.68%）。相比之下，NO 还原的半抑制浓度（IC）分别为 7.69% v/v（ABJ）和 24.48mM（阿司匹林）。与促炎对照（LPS）相比，ABJ 降低了 IL-1β、MCP-1 和 GCSF；阿司匹林降低了 IL1R1、MCP-1、GMCSF、GCSF 和 TNF-α；ABJ+阿司匹林治疗降低了 IL1R、GMCSF 和 CXCL10。细胞因子的计算机相互作用和潜在结合相互作用的预测表明，CCR1、CCR5 和 NF-kB 调节。这些结果表明，未充分利用的南美浆果具有抗炎潜力，并且与阿司匹林等已知药物联合应用可以减轻炎症相关疾病。这是首次报道 V. meridionale Swartz 果汁与阿司匹林联合应用对 LPS 刺激的 RAW 264.7 巨噬细胞的抗炎作用。
