Agudelo Carlos Daniel, Luzardo-Ocampo Iván, Hernández-Arriaga Angélica María, Rendón Julio César, Campos-Vega Rocio, Maldonado-Celis Maria Elena
School of Nutrition and Dietetics, University of Antioquia, Medellín, Antioquia 1226, Colombia.
Postgraduate Program in Food of the Center of the Republic (PROPAC), Research and Graduate Studies in Food Science, School of Chemistry, Autonomous University of Queretaro, Santiago de Querétaro, Qro 76010, Mexico.
Prev Nutr Food Sci. 2020 Sep 30;25(3):272-279. doi: 10.3746/pnf.2020.25.3.272.
Swartz, known as Andean berry, has a high content of anthocyanins, phenolic acids, and other flavonoids due to their putative anticancer activity. However, after consumption, the structures and function of these molecules may be altered. The purpose of this study was to evaluate the pro-apoptotic effect of fermented non-digestible fraction (FNDF) of Andean berry juice (ABJ) on colon adenocarcinoma HT29 cells. HT29 cells were treated by FNDF-ABJ obtained by gastrointestinal fermentation. We determined the proapoptotic capacity by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays, oxidative stress by analyzing superoxide dismutase and catalase activity, lipid peroxidation by measuring 8-iso-prostaglandin F2α, and measured lactate dehydrogenase. Our results show that FNDF-ABJ inhibited cell growth [lethal dose 50(%)=26% v/v]. In addition, FNDF-ABJ increased the number of TUNEL positive cells 2-fold compared with untreated cells without altering the release of lactate dehydrogenase. However, superoxide dismutase activity was reduced in HT29 cells treated with FNDF-ABJ, catalase activity was not affected and 8-iso-prostaglandin F2α levels were increased. These results support that the anti-proliferative effects of FNDF-ABJ on HT29 cells can be explained by apoptotic mechanisms.
斯沃茨，又称安第斯浆果，因其假定的抗癌活性而含有高含量的花青素、酚酸和其他类黄酮。然而，食用后，这些分子的结构和功能可能会发生改变。本研究的目的是评估安第斯浆果汁（ABJ）的发酵不可消化部分（FNDF）对结肠腺癌HT29细胞的促凋亡作用。HT29细胞用通过胃肠道发酵获得的FNDF-ABJ处理。我们通过末端脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL）测定法确定促凋亡能力，通过分析超氧化物歧化酶和过氧化氢酶活性来测定氧化应激，通过测量8-异前列腺素F2α来测定脂质过氧化，并测定乳酸脱氢酶。我们的结果表明，FNDF-ABJ抑制细胞生长[半数致死剂量（%）=26% v/v]。此外，与未处理的细胞相比，FNDF-ABJ使TUNEL阳性细胞数量增加了2倍，同时不改变乳酸脱氢酶的释放。然而，用FNDF-ABJ处理的HT29细胞中超氧化物歧化酶活性降低，过氧化氢酶活性未受影响，8-异前列腺素F2α水平升高。这些结果支持FNDF-ABJ对HT29细胞的抗增殖作用可通过凋亡机制来解释。
