Department of Biological Sciences, University of Alberta, Edmonton, AB T6G 2E9, Canada.
Ottawa Institute of Systems Biology, Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON K1H 8M5, Canada.
Viruses. 2018 Jun 16;10(6):332. doi: 10.3390/v10060332.
is a frequent foodborne pathogen of humans. As infections commonly arise from contaminated poultry, phage treatments have been proposed to reduce the load on farms to prevent human infections. While a prior report documented the transcriptome of phages during the carrier state life cycle, transcriptomic analysis of a lytic phage infection has not been reported. We used RNA-sequencing to profile the infection of NCTC 11168 by the lytic T4-like myovirus NCTC 12673. Interestingly, we found that the most highly upregulated host genes upon infection make up an uncharacterized operon (), which includes genes with similarity to T4 superinfection exclusion and antitoxin genes. Other significantly upregulated genes include those involved in oxidative stress defense and the multidrug efflux pump (CmeABC). We found that phage infectivity is altered by mutagenesis of the oxidative stress defense genes catalase (), alkyl-hydroxyperoxidase (), and superoxide dismutase (), and by mutagenesis of the efflux pump genes and . This suggests a role for these gene products in phage infection. Together, our results shed light on the phage-host dynamics of an important foodborne pathogen during lytic infection by a T4-like phage.
是人类中常见的食源性病原体。由于感染通常源自受污染的家禽，因此已提出噬菌体处理方法来降低农场中的负荷，以预防人类感染。虽然之前有一份报告记录了载体状态生命周期中噬菌体的转录组，但尚未报道裂解噬菌体感染的转录组分析。我们使用 RNA 测序对裂解 T4 样肌病毒 NCTC 12673 感染 NCTC 11168 进行了分析。有趣的是，我们发现感染后宿主基因上调幅度最大的是一个未被描述的操纵子()，其中包括与 T4 超感染排除和抗毒素基因具有相似性的基因。其他显著上调的基因包括参与氧化应激防御和多药外排泵 (CmeABC) 的基因。我们发现，通过对氧化应激防御基因过氧化氢酶 ()、烷基过氧化物酶 () 和超氧化物歧化酶 () 以及外排泵基因 和 的突变，噬菌体的感染力发生了改变。这表明这些基因产物在噬菌体感染中起作用。总之，我们的研究结果阐明了在 T4 样噬菌体的裂解感染期间，一种重要食源性病原体的噬菌体-宿主动态。
