Colbère-Garapin F, Horodniceanu F, Kourilsky P, Garapin A C
Unité de Virologie Médicale, Institut Pasteur, Paris, France.
EMBO J. 1983;2(1):21-5. doi: 10.1002/j.1460-2075.1983.tb01374.x.
The expression of human hepatitis B virus (HBV) surface (HBS) and e (HBe) antigens has been studied comparatively in monkey and mouse cell lines co-transfected with HBV DNA and the dominant selective marker aminoglycoside 3'-phosphotransferase gene. We have found that the kinetics and stability of expression of the HBS gene varies with the cell lines used. Only a late transient expression of both HBS and HBe is observed between 1 and 5 weeks after transfection in monkey kidney Vero cells transfected with the complete HBV genome, while a permanent expression of HBS and HBe is obtained in mouse cells. HBS and HBe are excreted into the cell culture medium. HBe is expressed in cells transfected with the complete HBV genome, but not with isolated HBS gene. In clones of Vero cells transformed with the HBS gene, HBV sequences were rearranged or lost.
在与乙肝病毒（HBV）DNA和显性选择标记氨基糖苷3'-磷酸转移酶基因共转染的猴和小鼠细胞系中，对人乙肝病毒表面（HBS）和e（HBe）抗原的表达进行了比较研究。我们发现，HBS基因表达的动力学和稳定性因所用细胞系而异。在用完整HBV基因组转染的猴肾Vero细胞中，转染后1至5周仅观察到HBS和HBe的晚期瞬时表达，而在小鼠细胞中则获得了HBS和HBe的永久表达。HBS和HBe分泌到细胞培养基中。HBe在转染完整HBV基因组的细胞中表达，但在转染分离的HBS基因的细胞中不表达。在用HBS基因转化的Vero细胞克隆中，HBV序列发生了重排或丢失。
