Hou Y T, Yang X K, Hu Y W
J Gen Virol. 1985 Aug;66 ( Pt 8):1819-23. doi: 10.1099/0022-1317-66-8-1819.
HindIII fragments D to P of DNA from a Chinese vaccine strain (Tian Tan) of vaccinia virus have been molecularly cloned into the plasmid pAT153 at the unique HindIII site. The Chinese strain DNA differs from a non-vaccine American strain (WR) in having an additional HindIII fragment (P). Twelve HindIII D clones and 12 HindIII F clones of the Chinese strain were analysed by digestion by EcoRI, BamHI, PstI and XhoI. Two forms of D (designated a and b) and of F (a and b) were demonstrated. In each the differences were detected as the presence of an additional EcoRI site, in Da and Fa. The HindIII Fa and Fb fragments of the Chinese strain were shown to differ significantly from the WR strain in their restriction site maps.
痘苗病毒中国疫苗株（天坛株）DNA的HindIII片段D至P已在质粒pAT153的唯一HindIII位点进行了分子克隆。中国株DNA与非疫苗美国株（WR）的不同之处在于有一个额外的HindIII片段（P）。通过EcoRI、BamHI、PstI和XhoI酶切分析了中国株的12个HindIII D克隆和12个HindIII F克隆。发现了D（命名为a和b）和F（a和b）的两种形式。在每种形式中，差异表现为在Da和Fa中存在一个额外的EcoRI位点。中国株的HindIII Fa和Fb片段在限制性酶切图谱上与WR株有显著差异。
