通过胆酸盐增溶法制备的细胞色素氧化酶囊泡晶体的三维重建。

Three-dimensional reconstruction of cytochrome oxidase vesicle crystals prepared by cholate solubilization.

作者信息

Kim C H, Radermacher M, Kessel M, Frank J, King T E

出版信息

J Inorg Biochem. 1985 Mar-Apr;23(3-4):163-9. doi: 10.1016/0162-0134(85)85021-2.

Abstract

Cytochrome oxidase vesicle crystals with long-range order have been obtained from cholate-solubilized, highly purified reconstitutively active preparations. These crystals, which are suitable for electron-microscopic structure investigation, show pgg symmetry in the 0 degree projection. Using Fourier reconstruction and modified back-projection methods, a three-dimensional reconstruction has been obtained at a resolution of 25 A. Our structural results are in agreement with the model of Henderson et al. [J. Mol. Biol. 112, 631 (1977)] obtained for their Triton-derived crystals.

摘要

已从经胆酸盐增溶的、高度纯化的重组活性制剂中获得具有长程有序的细胞色素氧化酶囊泡晶体。这些适合进行电子显微镜结构研究的晶体，在0度投影中显示出pgg对称性。使用傅里叶重建和改进的反投影方法，已获得分辨率为25埃的三维重建结果。我们的结构结果与亨德森等人[《分子生物学杂志》112, 631 (1977)]从其Triton衍生晶体获得的模型一致。