Department of Pharmaceutical Sciences, University of Perugia, Via del Giochetto, 06122, Perugia, Italy.
Department of Medical and Surgical Specialties, Radiological Science and Public Health, University of Brescia, 11 Viale Europa, 25123, Brescia, Italy.
Int J Hyg Environ Health. 2018 Jul;221(6):883-892. doi: 10.1016/j.ijheh.2018.05.014. Epub 2018 Jun 18.
Recent data support the hypothesis that genetic damage occurring early in life during childhood can play an important role in the development of chronic diseases in adulthood, including cancer.
The objective of this paper, part of the MAPEC_LIFE project, is to describe the frequency of micronuclei and meta-nuclear alterations in exfoliated buccal cells of 6-8year-old Italian children recruited in five Italian towns (i.e., Brescia, Torino, Pisa, Perugia and Lecce) with different air pollution levels.
About 200 children per town were recruited from primary schools. Biological samples were collected twice from the same children, in two different seasons (winter 2014-15 and late spring 2015). Cytogenetic damage was evaluated by the buccal micronucleus cytome assay.
Overall,n = 1046 children represent the final cohort of the MAPEC_LIFE study. On the whole, the results showed a higher mean MN frequency in winter (0.42 ± 0.54‰) than late-spring (0.22 ± 0.34‰). MN frequency observed among the five Italian towns showed a trend that follows broadly the levels of air pollution in Italy: the highest MN frequency was observed in Brescia during both seasons, the lowest in Lecce (winter) and Perugia (late-spring).
To the best of our knowledge, the number of recruited children included in the analysis (n = 1046) is the highest compared to previous studies evaluating the frequency of MN in exfoliated buccal cells so far. MN frequency was associated with winter season and living in towns at various levels of air pollution, suggesting an important role of this exposure in determining early cytogenetic effects.
最近的数据支持这样一种假设，即在儿童期早期发生的遗传损伤可能在成年期慢性疾病的发展中发挥重要作用，包括癌症。
本文是 MAPEC_LIFE 项目的一部分，旨在描述意大利五个城镇（布雷西亚、都灵、比萨、佩鲁贾和莱切）的 6-8 岁儿童脱落口腔细胞中的微核和多核改变的频率，这些城镇的空气污染水平不同。
每个城镇从小学招募了大约 200 名儿童。在两个不同的季节（2014-15 年冬季和 2015 年晚春），从同一儿童身上采集了两次生物样本。通过口腔微核细胞遗传学分析评估细胞遗传损伤。
总体而言，1046 名儿童代表了 MAPEC_LIFE 研究的最终队列。总的来说，冬季（0.42±0.54‰）的平均 MN 频率高于晚春（0.22±0.34‰）。意大利五个城镇的 MN 频率显示出一个趋势，大致反映了意大利的空气污染水平：在两个季节中，MN 频率最高的是布雷西亚，而最低的是莱切（冬季）和佩鲁贾（晚春）。
据我们所知，与迄今为止评估脱落口腔细胞中 MN 频率的先前研究相比，本次分析中纳入的被招募儿童的数量（n=1046）是最高的。MN 频率与冬季季节和生活在不同空气污染水平的城镇有关，表明这种暴露在决定早期细胞遗传效应方面起着重要作用。
