对从两例1型单纯疱疹病毒感染致死病例不同部位分离出的病毒的DNA进行限制性内切酶消化分析。
Restriction endonuclease digestion analysis of DNA from viruses isolated from different sites of two fatal cases of herpes simplex virus type-1 infection.
作者信息
McFarlane E S, Embil J A, Murphy D M, Krause V W
出版信息
J Med Microbiol. 1985 Aug;20(1):27-32. doi: 10.1099/00222615-20-1-27.
Herpes simplex virus (HSV) type-1 was isolated from a fatal case of herpes simplex encephalitis (case 1) and from a fatal case of disseminated herpes simplex (case 2). The virus was isolated from the lip lesion, the frontal lobe and the temporal lobe of the brain in case 1 and from a mesenteric node, myocardium and salivary gland in case 2. Restriction endonuclease digestion analysis showed that each case was infected with different substrains of HSV. The changes in band pattern in isolates from case 1 occurred in the "variable" region of the genome, showing that viruses with such variations can be isolated simultaneously from different tissues. The changes in band patterns in isolates from case 2 indicated the presence of two virus substrains, one in the mesenteric node and salivary gland and a second in the myocardium.
1型单纯疱疹病毒是从一例单纯疱疹性脑炎死亡病例（病例1）和一例播散性单纯疱疹死亡病例（病例2）中分离出来的。在病例1中，病毒是从唇部病变、额叶和颞叶中分离出来的；在病例2中，病毒是从肠系膜淋巴结、心肌和唾液腺中分离出来的。限制性内切酶消化分析表明，每个病例感染的是单纯疱疹病毒的不同亚株。病例1分离株的条带模式变化发生在基因组的“可变”区域，这表明具有这种变异的病毒可以同时从不同组织中分离出来。病例2分离株的条带模式变化表明存在两种病毒亚株，一种存在于肠系膜淋巴结和唾液腺中，另一种存在于心肌中。
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