Chaney S M, Warren K G, Subak-Sharpe J H
J Gen Virol. 1983 Dec;64 ( Pt 12):2717-33. doi: 10.1099/0022-1317-64-12-2717.
The distribution of restriction endonuclease (RE) sites was compared for 84 herpes simplex virus type 1 (HSV-1) isolates obtained from the ganglia, facial lesions, genital lesions and from brain tissue from herpes encephalitis cases. The isolates came from Canada, the U.K., the U.S.A. and Japan. Out of a total of 224 sites identified, 87 were variable. Three of the 30 most variable sites were at significantly (P less than 0.05) different frequencies in groups of isolates from distinct anatomical sites of isolation; one of these, and a further two sites, were at significantly different frequencies in groups from distinct geographical origins. There are at least two inter-related linkage groups. However, most of the site combinations appear to be random. The variability of RE sites in contiguous genome segments, which include both non-coding and coding sequences, show a marked heterogeneity, indicating that some viral gene sequences are more variable than others. The three RE sites at different frequencies in viral groups from distinct anatomical sites of isolation are in two genome segments: map units 27 to 35 and 50 to 57. We infer from the observed associations with anatomical site of viral isolation that part of at least one of these segments may modulate viral virulence in man following infection.
对从神经节、面部病变、生殖器病变以及疱疹性脑炎病例的脑组织中分离得到的84株1型单纯疱疹病毒（HSV-1）的限制性内切酶（RE）位点分布进行了比较。这些分离株来自加拿大、英国、美国和日本。在总共鉴定出的224个位点中，有87个是可变的。30个变化最大的位点中有3个在来自不同解剖学分离部位的分离株组中频率显著不同（P小于0.05）；其中一个位点以及另外两个位点在来自不同地理起源的组中频率显著不同。至少有两个相互关联的连锁群。然而，大多数位点组合似乎是随机的。连续基因组片段中RE位点的变异性，包括非编码和编码序列，显示出明显的异质性，表明一些病毒基因序列比其他序列更具变异性。在来自不同解剖学分离部位的病毒组中频率不同的三个RE位点位于两个基因组片段中：图谱单位27至35和50至57。我们从观察到的与病毒分离的解剖学部位的关联推断，这些片段中至少有一个片段的一部分可能在感染后调节人类的病毒毒力。
