Moore G R, Robinson M N, Williams G, Williams R J
J Mol Biol. 1985 Jun 5;183(3):429-46. doi: 10.1016/0022-2836(85)90012-9.
The 1H nuclear magnetic resonance spectrum of tuna ferrocytochrome c has been studied and the resonances of all 49 amino acid methyl groups have been assigned to specific absorption lines. In comparison with resonance assignments in the ferricytochrome c spectrum, the secondary shifts of resonances of ferrocytochrome c are smaller and the identification of characteristic spin-systems from comparison of spectra from homologous proteins more difficult. For this reason, two-dimensional nuclear magnetic resonance exchange correlated spectroscopy has been used to correlate the assigned resonances of tuna ferricytochrome c with previously unassigned resonances of tuna ferrocytochrome c.
对金枪鱼亚铁细胞色素c的1H核磁共振谱进行了研究，已将所有49个氨基酸甲基的共振峰归属到特定的吸收线上。与高铁细胞色素c谱中的共振峰归属相比，亚铁细胞色素c共振峰的二级位移较小，且从同源蛋白质光谱比较中鉴定特征自旋系统更加困难。因此，二维核磁共振交换相关光谱已被用于将金枪鱼高铁细胞色素c已归属的共振峰与金枪鱼亚铁细胞色素c先前未归属的共振峰进行关联。
