Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor Darul Ehsan, Malaysia.
Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor Darul Ehsan, Malaysia.
Appl Biochem Biotechnol. 2019 Jan;187(1):211-220. doi: 10.1007/s12010-018-2807-2. Epub 2018 Jun 19.
Bio-cellulose is the microbial extracellular cellulose that is produced by growing several microorganisms on agriculture by-products, and it is used in several food applications. This study aims to utilize sago by-product, coconut water, and the standard medium Hestrin-Schramm as the carbon sources in the culture medium for bio-cellulose production. The bacteria Beijerinkia fluminensis WAUPM53 and Gluconacetobacter xylinus 0416 were selected based on their bio-cellulose production activity. The structure was determined by Fourier transform infrared spectroscopy and scanning electron microscopy, while the toxicity safety was evaluated by brine shrimp lethality test. The results of Fourier transform infrared spectroscopy showed that the bio-cellulose produced by B. fluminensis cultivated in sago by-products was of high quality. The bio-cellulose production by B. fluminensis in the sago by-product medium was slightly higher than that in the coconut water medium and was comparable with the production in the Hestrin-Schramm medium. Brine shrimp lethality test confirmed that the bio-cellulose produced by B. fluminensis in the sago by-product medium has no toxicity, which is safe for applications in the food industry. This is the first study to determine the high potential of sago by-product to be used as a new carbon source for the bio-cellulose production.
生物纤维素是由几种微生物在农业副产品上生长产生的微生物细胞外纤维素，它被用于多种食品应用。本研究旨在利用西米副产品、椰子水和标准培养基 Hestrin-Schramm 作为生物纤维素生产培养基中的碳源。根据生物纤维素生产活性选择了 Beijerinkia fluminensis WAUPM53 和 Gluconacetobacter xylinus 0416 两种细菌。通过傅里叶变换红外光谱和扫描电子显微镜确定结构，通过盐水虾致死试验评估毒性安全性。傅里叶变换红外光谱的结果表明，由 B. fluminensis 在西米副产品中培养的生物纤维素质量很高。B. fluminensis 在西米副产品培养基中的生物纤维素产量略高于椰子水培养基中的产量，与 Hestrin-Schramm 培养基中的产量相当。盐水虾致死试验证实，由 B. fluminensis 在西米副产品培养基中产生的生物纤维素没有毒性，可安全应用于食品工业。这是首次确定西米副产品具有作为生物纤维素生产新碳源的巨大潜力的研究。
