Çevirme Deniz, Adademir Taylan, Kafalı Başaran Eylül, Şavluk Ömer Faruk, Elibol Ahmet, Erkanlı Şentürk Gözde, Akkaya Hatice, Yılmaz Bayram
Turk J Med Sci. 2018 Jun 14;48(3):661-669. doi: 10.3906/sag-1710-74.
Background/aim: Alprostadil and iloprost are successful agents used for both pulmonary hypertension and extremity ischemia treatment. Different systemic effects of these agents may change the preferences of clinical usage. Superiority of preventing ischemia/ reperfusion (IR) injury is a criterion for clinical preference of these agents. The present study was designed to compare the protective effects of alprostadil and iloprost in a rat model of IR injury. Materials and methods: Twenty-three male Sprague Dawley rats were used (aged 8-12 weeks, mean weight 230 ± 30 g). They were randomized into 4 groups: Group 1 (iloprost + IR), Group 2 (alprostadil + IR), Group 3 (saline + IR), and Group 4 (control). Under general anesthesia, in all groups except Group 4, the abdominal region was explored and the abdominal aorta was temporarily clamped for 60 min. After the clamp was removed, 120 min of reperfusion was applied. In Group 4, the rats were placed under general anesthesia and abdominal exploration was performed without the IR procedure. For all rats, body temperature was kept at 36 °C with a heater pad through the whole procedure. The rats were euthanized under general anesthesia to remove the kidneys and lungs for study. Histopathological and biochemical analyses were conducted with kidney and lung tissues. Histopathological scoring was done by analyzing cellular damage at tissue level. Malondialdehyde (MDA), superoxide dismutase, and glutathione levels were studied for biochemical analysis. Results: Histopathologic analysis showed that, as compared with alprostadil, iloprost provided a significantly higher level of renal protection against IR injury (P < 0.01). Renal tissue levels of MDA were significantly lower in the alprostadil group as compared to Group 3 (P < 0.05). Conclusion: Alprostadil and iloprost seem to provide protection against IR injury, with iloprost being more protective in renal tissue. Alprostadil is more effective than iloprost in protecting lung tissue against IR injury.
背景/目的：前列地尔和伊洛前列素是用于治疗肺动脉高压和肢体缺血的有效药物。这些药物不同的全身效应可能会改变临床使用的偏好。预防缺血/再灌注（IR）损伤的优越性是这些药物临床偏好的一个标准。本研究旨在比较前列地尔和伊洛前列素在大鼠IR损伤模型中的保护作用。
使用23只雄性Sprague Dawley大鼠（8 - 12周龄，平均体重230±30 g）。它们被随机分为4组：第1组（伊洛前列素+IR）、第2组（前列地尔+IR）、第3组（生理盐水+IR）和第4组（对照组）。在全身麻醉下，除第4组外，所有组均探查腹部并暂时夹闭腹主动脉60分钟。夹闭去除后，进行120分钟的再灌注。在第4组中，大鼠接受全身麻醉但不进行IR操作，仅进行腹部探查。对于所有大鼠，整个过程中使用加热垫将体温保持在36℃。大鼠在全身麻醉下安乐死，取出肾脏和肺进行研究。对肾脏和肺组织进行组织病理学和生化分析。通过分析组织水平的细胞损伤进行组织病理学评分。研究丙二醛（MDA）、超氧化物歧化酶和谷胱甘肽水平进行生化分析。
组织病理学分析表明，与前列地尔相比，伊洛前列素对IR损伤提供了显著更高水平的肾脏保护（P < 0.01）。与第3组相比，前列地尔组的肾组织MDA水平显著降低（P < 0.05）。
前列地尔和伊洛前列素似乎都能提供抗IR损伤的保护作用，伊洛前列素在肾组织中更具保护作用。前列地尔在保护肺组织免受IR损伤方面比伊洛前列素更有效。
