1 Central Diagnostic Laboratory, Cluster for Hemostasis and Transfusion, Maastricht University Medical Center (MUMC+), Maastricht, the Netherlands.
Clin Appl Thromb Hemost. 2019 Jan-Dec;25:1076029618782445. doi: 10.1177/1076029618782445. Epub 2018 Jun 19.
Platelet aggregation can be measured using optical aggregation (light transmission aggregometry, LTA) as well as by impedance (Multiplate analyzer). The LTA (the gold standard method) can be influenced by many preanalytical variables. Several guidelines differ in recommendations for the duration patients should refrain from smoking, coffee, fatty meals, and physical exercise prior to blood collection for performing platelet function tests. In this pilot study, the influence of smoking, coffee, high-fat meal, or physical exercise on platelet aggregation was investigated to improve patient friendliness and laboratory logistics in platelet function diagnostics. Standardized blood collection was performed when participants were fasting and after each parameter (n=5 per group). As a control for diurnal fluctuations, participants (n=6) were fasting during both blood collections. Platelet aggregation was executed using standardized methods for LTA and Multiplate analyzer. Statistical analysis of the results using Wilcoxon signed-rank test did not show any significant differences in platelet aggregation in healthy participants under different preanalytical variables. Therefore, these variables are not expected to adversely affect testing, which can avoid canceling tests for those patients who inevitably did.
血小板聚集可以通过光学聚集（透光率聚集测定法，LTA）以及阻抗（多板分析仪）来测量。LTA（金标准方法）可能受到许多分析前变量的影响。一些指南在建议患者在进行血小板功能测试前应避免吸烟、咖啡、高脂肪餐和体育锻炼的时间上存在差异。在这项初步研究中，研究了吸烟、咖啡、高脂肪餐或体育锻炼对血小板聚集的影响，以改善患者友好度和血小板功能诊断实验室的后勤工作。在参与者禁食和每个参数后（每组 5 人）进行标准化血液采集（n=5 每一组）。为了控制昼夜波动，参与者（n=6）在两次采血期间均禁食。使用标准化方法进行 LTA 和多板分析仪的血小板聚集实验。使用 Wilcoxon 符号秩检验对结果进行统计学分析，未显示不同分析前变量下健康参与者的血小板聚集有任何显著差异。因此，这些变量预计不会对检测产生不利影响，从而避免取消不可避免的患者的检测。
