• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于 RNA 测序分析的人下咽鳞状细胞癌中异常表达的基因和 miRNAs。

Aberrantly expressed genes and miRNAs in human hypopharyngeal squamous cell carcinoma based on RNA‑sequencing analysis.

机构信息

Department of Otolaryngology, Head and Neck Surgery, The First People's Hospital of Jining, Jining, Shandong 272000, P.R. China.

Department of Obstetrics, The First Maternity and Child Health Hospital of Jining, Jining, Shandong 272000, P.R. China.

出版信息

Oncol Rep. 2018 Aug;40(2):647-658. doi: 10.3892/or.2018.6506. Epub 2018 Jun 19.

DOI:10.3892/or.2018.6506
PMID:29916534
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6072292/
Abstract

The aim of the present study was to investigate the key genes, miRNAs and pathways in hypopharyngeal squamous cell carcinoma (HPSCC) and to elucidate the mechanisms underlying HPSCC development. The gene and microRNA (miRNA) expression profiles of HPSCC tissues and adjacent normal tissues from three subjects were obtained. Differentially expressed genes (DEGs) and differentially expressed miRNAs were identified in HPSCC. Functional annotation and protein‑protein interaction (PPI) network were conducted to elucidate the biological functions of DEGs. A total of 160 DEGs (16 upregulated and 144 downregulated genes) and 79 differentially expressed miRNAs (48 upregulated and 31 downregulated miRNAs) were identified in HPSCC. The deregulated genes were significantly involved in spliceosome, cell cycle and RNA degradation. In the PPI network, S‑phase kinase associated protein 1 (SKP1), non‑POU domain containing octamer binding (NONO) and zinc activated ion channel (ZACN) were identified as hub proteins. On the whole, the present study may help to gain a comprehensive understanding of tumorigenesis in HPSCC and provide valuable information for early diagnosis and drug design of HPSCC in future research.

摘要

本研究旨在探讨下咽鳞状细胞癌（HPSCC）中的关键基因、微小 RNA（miRNA）和途径，并阐明 HPSCC 发展的机制。本研究从三个受试者中获得了 HPSCC 组织和相邻正常组织的基因和 miRNA 表达谱。鉴定了 HPSCC 中的差异表达基因（DEGs）和差异表达 miRNA。对 DEGs 进行了功能注释和蛋白质-蛋白质相互作用（PPI）网络分析，以阐明 DEGs 的生物学功能。在 HPSCC 中鉴定出 160 个差异表达基因（16 个上调和 144 个下调基因）和 79 个差异表达 miRNA（48 个上调和 31 个下调 miRNA）。失调基因显著参与剪接体、细胞周期和 RNA 降解。在 PPI 网络中，鉴定出 S 期激酶相关蛋白 1（SKP1）、非 POUS 域包含八聚体结合（NONO）和锌激活离子通道（ZACN）作为枢纽蛋白。总的来说，本研究可能有助于全面了解 HPSCC 的肿瘤发生，并为未来研究中 HPSCC 的早期诊断和药物设计提供有价值的信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a5a/6072292/b9c6831d8ed0/OR-40-02-0647-g08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a5a/6072292/9c5979715575/OR-40-02-0647-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a5a/6072292/2ca75848a000/OR-40-02-0647-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a5a/6072292/27b47e834f8f/OR-40-02-0647-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a5a/6072292/6df5fe319041/OR-40-02-0647-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a5a/6072292/be5ddfdb753c/OR-40-02-0647-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a5a/6072292/ecc412f2534b/OR-40-02-0647-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a5a/6072292/7f8c8ddf57e1/OR-40-02-0647-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a5a/6072292/f76b0df65398/OR-40-02-0647-g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a5a/6072292/b9c6831d8ed0/OR-40-02-0647-g08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a5a/6072292/9c5979715575/OR-40-02-0647-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a5a/6072292/2ca75848a000/OR-40-02-0647-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a5a/6072292/27b47e834f8f/OR-40-02-0647-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a5a/6072292/6df5fe319041/OR-40-02-0647-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a5a/6072292/be5ddfdb753c/OR-40-02-0647-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a5a/6072292/ecc412f2534b/OR-40-02-0647-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a5a/6072292/7f8c8ddf57e1/OR-40-02-0647-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a5a/6072292/f76b0df65398/OR-40-02-0647-g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a5a/6072292/b9c6831d8ed0/OR-40-02-0647-g08.jpg

相似文献

1
Aberrantly expressed genes and miRNAs in human hypopharyngeal squamous cell carcinoma based on RNA‑sequencing analysis.基于 RNA 测序分析的人下咽鳞状细胞癌中异常表达的基因和 miRNAs。
Oncol Rep. 2018 Aug;40(2):647-658. doi: 10.3892/or.2018.6506. Epub 2018 Jun 19.
2
Molecular mechanisms of pathogenesis in hepatocellular carcinoma revealed by RNA‑sequencing.RNA 测序揭示肝细胞癌发病机制的分子机制。
Mol Med Rep. 2017 Nov;16(5):6674-6682. doi: 10.3892/mmr.2017.7457. Epub 2017 Sep 11.
3
Effect of microRNA-203 on tumor growth in human hypopharyngeal squamous cell carcinoma.微小RNA-203对人下咽鳞状细胞癌肿瘤生长的影响
Mol Cell Biochem. 2015 Jul;405(1-2):97-104. doi: 10.1007/s11010-015-2401-7. Epub 2015 Apr 4.
4
Oral squamous cell carcinoma: microRNA expression profiling and integrative analyses for elucidation of tumourigenesis mechanism.口腔鳞状细胞癌：用于阐明肿瘤发生机制的微小RNA表达谱分析及综合分析
Mol Cancer. 2016 Apr 7;15:28. doi: 10.1186/s12943-016-0512-8.
5
Gene expression profiling via bioinformatics analysis reveals biomarkers in laryngeal squamous cell carcinoma.通过生物信息学分析进行基因表达谱分析揭示了喉鳞状细胞癌中的生物标志物。
Mol Med Rep. 2015 Aug;12(2):2457-64. doi: 10.3892/mmr.2015.3701. Epub 2015 Apr 29.
6
Identification of Key Genes and Pathways in Tongue Squamous Cell Carcinoma Using Bioinformatics Analysis.基于生物信息学分析的舌鳞状细胞癌关键基因和通路的鉴定。
Med Sci Monit. 2017 Dec 14;23:5924-5932. doi: 10.12659/msm.905035.
7
Biomarker identification in clear cell renal cell carcinoma based on miRNA-seq and digital gene expression-seq data.基于miRNA测序和数字基因表达测序数据的透明细胞肾细胞癌生物标志物鉴定
Gene. 2018 Mar 20;647:205-212. doi: 10.1016/j.gene.2017.12.031. Epub 2017 Dec 15.
8
Bioinformatics analysis of gene expression profiles of esophageal squamous cell carcinoma.食管鳞状细胞癌基因表达谱的生物信息学分析
Dis Esophagus. 2017 May 1;30(5):1-8. doi: 10.1093/dote/dow018.
9
Exploring the molecular mechanisms of osteosarcoma by the integrated analysis of mRNAs and miRNA microarrays.通过 mRNA 和 miRNA 芯片的综合分析探索骨肉瘤的分子机制。
Int J Mol Med. 2018 Jul;42(1):21-30. doi: 10.3892/ijmm.2018.3594. Epub 2018 Mar 27.
10
MicroRNAs function as tumor suppressors or oncogenes: aberrant expression of microRNAs in head and neck squamous cell carcinoma.微小RNA发挥肿瘤抑制因子或癌基因的作用：头颈部鳞状细胞癌中微小RNA的异常表达
Auris Nasus Larynx. 2013 Apr;40(2):143-9. doi: 10.1016/j.anl.2012.07.001. Epub 2012 Jul 24.

引用本文的文献

1
ZACN Associated with Poor Prognosis Promotes Proliferation of Kidney Renal Clear Cell Carcinoma Cells by Inhibiting JTC801-Induced Alkaliptosis.与不良预后相关的ZACN通过抑制JTC801诱导的碱中毒促进肾透明细胞癌细胞增殖。
Appl Biochem Biotechnol. 2025 May;197(5):3346-3362. doi: 10.1007/s12010-025-05197-1. Epub 2025 Feb 12.
2
Exosomal microRNAs Targeting Gene as Promising Prognostic Markers for Head and Neck Squamous Cell Carcinoma.靶向基因的外泌体微小RNA作为头颈部鳞状细胞癌有前景的预后标志物
Glob Med Genet. 2022 Dec 14;9(4):277-286. doi: 10.1055/s-0042-1758204. eCollection 2022 Dec.
3
Delineation of the functional properties exhibited by the Zinc-Activated Channel (ZAC) and its high-frequency ThrAla variant (rs2257020) in Xenopus oocytes.

本文引用的文献

1
Robust expression of tumor suppressor miRNA's let-7 and miR-195 detected in plasma of Saudi female breast cancer patients.在沙特女性乳腺癌患者的血浆中检测到肿瘤抑制 miRNA let-7 和 miR-195 的稳健表达。
BMC Cancer. 2017 Nov 28;17(1):799. doi: 10.1186/s12885-017-3776-5.
2
Infrequently expressed miRNAs influence survival after diagnosis with colorectal cancer.表达频率较低的微小RNA影响结直肠癌诊断后的生存率。
Oncotarget. 2017 Aug 3;8(48):83845-83859. doi: 10.18632/oncotarget.19863. eCollection 2017 Oct 13.
3
MiR-15a-5p negatively regulates cell survival and metastasis by targeting CXCL10 in chronic myeloid leukemia.
锌激活通道（ZAC）及其高频率 ThrAla 变体（rs2257020）在非洲爪蟾卵母细胞中表现出的功能特性的描述。
Pharmacol Res. 2021 Jul;169:105653. doi: 10.1016/j.phrs.2021.105653. Epub 2021 May 4.
4
Ion Channel Dysregulation in Head and Neck Cancers: Perspectives for Clinical Application.离子通道失调与头颈部癌症：临床应用展望。
Rev Physiol Biochem Pharmacol. 2021;181:375-427. doi: 10.1007/112_2020_38.
5
Two miRNA prognostic signatures of head and neck squamous cell carcinoma: A bioinformatic analysis based on the TCGA dataset.头颈部鳞状细胞癌的两种miRNA预后特征：基于TCGA数据集的生物信息学分析
Cancer Med. 2020 Apr;9(8):2631-2642. doi: 10.1002/cam4.2915. Epub 2020 Feb 17.
在慢性髓性白血病中，微小RNA-15a-5p通过靶向趋化因子配体10负向调节细胞存活和转移。
Am J Transl Res. 2017 Sep 15;9(9):4308-4316. eCollection 2017.
4
miR-125b-5p functions as a tumor suppressor gene partially by regulating HMGA2 in esophageal squamous cell carcinoma.miR-125b-5p在食管鳞状细胞癌中部分通过调控HMGA2发挥肿瘤抑制基因的作用。
PLoS One. 2017 Oct 2;12(10):e0185636. doi: 10.1371/journal.pone.0185636. eCollection 2017.
5
MicroRNA differential expression spectrum and microRNA-125a-5p inhibition of laryngeal cancer cell proliferation.微小RNA差异表达谱及微小RNA-125a-5p对喉癌细胞增殖的抑制作用
Exp Ther Med. 2017 Aug;14(2):1699-1705. doi: 10.3892/etm.2017.4685. Epub 2017 Jun 27.
6
LncRNA-TCONS_00026907 is involved in the progression and prognosis of cervical cancer through inhibiting miR-143-5p.长链非编码 RNA-TCONS_00026907 通过抑制 miR-143-5p 参与宫颈癌的进展和预后。
Cancer Med. 2017 Jun;6(6):1409-1423. doi: 10.1002/cam4.1084. Epub 2017 May 23.
7
UNR/CSDE1 Drives a Post-transcriptional Program to Promote Melanoma Invasion and Metastasis.UNR/CSDE1 驱动一个转录后程序，促进黑色素瘤侵袭和转移。
Cancer Cell. 2016 Nov 14;30(5):694-707. doi: 10.1016/j.ccell.2016.10.004. Epub 2016 Oct 27.
8
Quality assurance of the gene ontology using abstraction networks.使用抽象网络对基因本体进行质量保证。
J Bioinform Comput Biol. 2016 Jun;14(3):1642001. doi: 10.1142/S0219720016420014. Epub 2015 Nov 24.
9
Long noncoding RNA GAPLINC promotes invasion in colorectal cancer by targeting SNAI2 through binding with PSF and NONO.长链非编码RNA GAPLINC通过与PSF和NONO结合靶向SNAI2促进结直肠癌侵袭。
Oncotarget. 2016 Jul 5;7(27):42183-42194. doi: 10.18632/oncotarget.9741.
10
Prognostic microRNA signatures derived from The Cancer Genome Atlas for head and neck squamous cell carcinomas.源自癌症基因组图谱的头颈部鳞状细胞癌预后微小RNA特征。
Cancer Med. 2016 Jul;5(7):1619-28. doi: 10.1002/cam4.718. Epub 2016 Apr 25.