Zinke Maximilian, Fricke Pascal, Lange Sascha, Zinn-Justin Sophie, Lange Adam
Department of Molecular Biophysics, Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany.
Institute for Integrative Biology of the Cell (I2BC) CEA, CNRS, Université Paris-Sud Université Paris-Saclay, Gif-sur-Yvette Cedex, France.
Chemphyschem. 2018 Oct 5;19(19):2457-2460. doi: 10.1002/cphc.201800542. Epub 2018 Jun 27.
Proton detection and fast magic-angle spinning have advanced biological solid-state NMR, allowing for the backbone assignment of complex protein assemblies with high sensitivity and resolution. However, so far no method has been proposed to detect intermolecular interfaces in these assemblies by proton detection. Herein, we introduce a concept based on methyl labeling that allows for the assignment of these moieties and for the study of protein-protein interfaces at atomic resolution.
质子检测和快速魔角旋转推动了生物固态核磁共振技术的发展，使得复杂蛋白质组装体的主链归属能够以高灵敏度和分辨率实现。然而，到目前为止，尚未有通过质子检测来检测这些组装体中分子间界面的方法被提出。在此，我们引入了一种基于甲基标记的概念，该概念能够实现这些基团的归属，并能在原子分辨率下研究蛋白质 - 蛋白质界面。
