Chang C C, Trosko J E, Kung H J, Bombick D, Matsumura F
Proc Natl Acad Sci U S A. 1985 Aug;82(16):5360-4. doi: 10.1073/pnas.82.16.5360.
The effects of the src gene on the activity of protein kinase C and intercellular communication have been studied in transformed NIH/3T3 clones isolated from soft agar following transfection with the plasmid carrying the v-src gene (psrc-11). Six transformed clones that were studied contained newly incorporated v-src genes in the genome, had an increased amount of pp60src, and showed enhanced activities of protein kinase C. Intercellular communication, studied by observing with autoradiography the transfer of [3H]uridine nucleotide from prelabeled donor cells to recipient cells in contact, was found to be reduced in transformed clones as compared to parental NIH/3T3 cells. Treatment with phorbol 12-myristate 13-acetate was also found to increase protein kinase C activity and to reduce intercellular communication in normal NIH/3T3 cells. These results suggest that the v-src gene product, in a manner similar to some of the powerful tumor promoters, may directly or indirectly affect cell-cell communication.
在转染携带v-src基因的质粒（psrc-11）后，从软琼脂中分离出转化的NIH/3T3克隆，研究了src基因对蛋白激酶C活性和细胞间通讯的影响。所研究的六个转化克隆在基因组中含有新整合的v-src基因，pp60src量增加，并且蛋白激酶C活性增强。通过放射自显影观察[3H]尿苷酸从预先标记的供体细胞向接触的受体细胞的转移来研究细胞间通讯，发现与亲本NIH/3T3细胞相比，转化克隆中的细胞间通讯减少。还发现用佛波醇12-肉豆蔻酸酯13-乙酸酯处理可增加正常NIH/3T3细胞中的蛋白激酶C活性并减少细胞间通讯。这些结果表明，v-src基因产物可能以类似于某些强大肿瘤启动子的方式直接或间接影响细胞间通讯。
