College of Bioengineering, Sichuan University of Science & Engineering, 180 Xueyuan Road, Zigong, Sichuan 643000, China.
Genome. 2018 Aug;61(8):605-613. doi: 10.1139/gen-2017-0177. Epub 2018 Jun 19.
Clostridium kluyveri is an anaerobic microorganism that is well-known for producing butyrate and hexanoate using ethanol and acetate. It is also an important bacterium in the production of Chinese strong flavour baijiu (SFB). To obtain a comprehensive understanding of its metabolism, a curated genome-scale metabolic model (GSMM) of C. kluyveri, including 708 genes, 994 reactions, and 804 metabolites, was constructed and named iCKL708. This model was used to simulate the growth of C. kluyveri on different carbon substrates and the results agreed well with the experimental data. The butyrate, pentanoate, and hexanoate biosynthesis pathways were also elucidated. Flux balance analysis indicated that the ratio of ethanol to acetate, as well as the uptake rate of carbon dioxide, affected hexanoate production. The GSMM iCKL708 described here provides a platform to further our understanding and exploration of the metabolic potential of C. kluyveri.
克氏梭菌是一种厌氧微生物，它以利用乙醇和乙酸生产丁酸和己酸而闻名。它也是中国浓香型白酒（SFB）生产中的一种重要细菌。为了全面了解其代谢，构建并命名了一个包含 708 个基因、994 个反应和 804 种代谢物的克氏梭菌精细化基因组尺度代谢模型（iCKL708）。该模型用于模拟克氏梭菌在不同碳底物上的生长，结果与实验数据吻合较好。此外，还阐明了丁酸、戊酸和己酸的生物合成途径。通量平衡分析表明，乙醇与乙酸的比例以及二氧化碳的摄取速率影响己酸的生成。这里描述的 iCKL708 基因组尺度代谢模型为进一步了解和探索克氏梭菌的代谢潜力提供了一个平台。
