Facultad de Ciencias de la Salud, Carrera de Enfermería, Grupo de Bio-Quimioinformática, Universidad de Las Américas (UDLA), Quito 170125, Ecuador.
Facultad de Ingeniería y Ciencias Aplicadas, Carrera de Ingeniería Agroindustrial, Universidad de Las Américas (UDLA), Quito 170125, Ecuador.
Molecules. 2023 Jan 10;28(2):693. doi: 10.3390/molecules28020693.
Horchata, a herbal infusion drink from Ecuador containing a mixture of medicinal plants, has been reported to exhibit anti-inflammatory, analgesic, diuretic, and antioxidant activity. The antibacterial activity of each of the plants contained in the horchata mixture has not been fully evaluated. Thus, in this study, we analysed the antibacterial activity of 21 plants used in horchata, collected from the Ecuadorian Andes region, against bacterial strains of clinical importance. The methanolic extract of sp. showed minimal inhibitory concentration (MIC) values of 250 µg/mL against ATCC25923 and Methicillin-resistant (MRSA), while exhibited a MIC value of 500 µg/mL towards ATCC25923. The high-performance liquid chromatography-diode array detector-tandem mass spectrometry (HPLC-DAD-MS/MS) analyses identified in sp. epicatechin tannins, cinnamaldehyde, and prehelminthosporol molecules, whereas in , gallocatechin and epigallocatechin tannins, some flavonoids, and gallic acid and derivatives were identified. Finally, sp. and showed partial inhibition of biofilm formation of ATCC25923 and MRSA. Overall, our findings revealed which of the plants used in horchata are responsible for the antibacterial activity attributed to this herbal drink and exhibit the potential for sp. and secondary metabolites to be explored as scaffolds in drug development.
来自厄瓜多尔的草药汤剂 horchata 由多种药用植物混合而成，具有抗炎、镇痛、利尿和抗氧化活性。然而，其中所含植物的抗菌活性尚未得到充分评估。因此，在这项研究中，我们分析了 horchata 中 21 种植物的抗菌活性，这些植物均来自厄瓜多尔安第斯山脉地区，针对的是临床重要的细菌菌株。 sp. 的甲醇提取物对 ATCC25923 和耐甲氧西林金黄色葡萄球菌（MRSA）的最小抑菌浓度（MIC）值为 250 µg/mL，而 对 ATCC25923 的 MIC 值为 500 µg/mL。高效液相色谱-二极管阵列检测器-串联质谱（HPLC-DAD-MS/MS）分析表明， sp. 中含有表儿茶素单宁、肉桂醛和前镰刀菌素分子，而 中则含有没食子酸单宁、儿茶素和表没食子儿茶素、一些类黄酮以及没食子酸和其衍生物。最后， sp. 和 对 ATCC25923 和 MRSA 的生物膜形成具有部分抑制作用。总的来说，我们的研究结果揭示了 horchata 中哪些植物成分具有抗菌活性，并发现 sp. 和 中的次生代谢产物具有作为药物开发的潜在支架的潜力。
