Department of Dental Hygiene, Gachon University College of Health Science, Republic of Korea.
Department of Preventive Dentistry & Public Oral Health, BK21 PLUS Project, Oral Science Research Institute, Yonsei University College of Dentistry, Republic of Korea.
Photodiagnosis Photodyn Ther. 2018 Sep;23:176-180. doi: 10.1016/j.pdpdt.2018.06.014. Epub 2018 Jun 18.
The aims of this study were to estimate the histological and optical changes of early carious lesions according to the duration of demineralization and to compare the efficacy to detect early carious lesions of a quantitative light-induced fluorescence (QLF) system with a spectrophotometer according to the lesion depth.
Eighty-four bovine enamel specimens were immersed in demineralizing gel for six distinct days. The severities of lesions were evaluated by lesion depths (Ld) by polarized light microscopy, fluorescence loss (ΔF) using QLF, and brightness (ΔL) by spectrophotometry. The relationships between Ld, ΔF, and ΔLwere analyzed.
Ld tended to be significantly deeper according to the demineralizing period (p < 0.05). The ΔF significantly decreased (p < 0.05) and the ΔL significantly increased with increasing the demineralizing period (p < 0.05). More shallow lesions (Ld≤200 μm) showed a strong relationship between ΔF and Ld (r = -0.898, p < 0.001), while there was a strong relationship between ΔL and Ld in deeper lesions (Ld>200 μm) (r = 0.858, p < 0.001). Our findings show that QLF system is a more efficient device for shallow early caries detection and that the spectrophotometer is a more stable device for the detection of deeper lesions.
本研究旨在根据脱矿持续时间评估早期龋损的组织学和光学变化，并根据病变深度比较定量光荧光（QLF）系统和分光光度计检测早期龋损的效果。
84 个牛牙釉质标本浸泡在脱矿凝胶中 6 天。通过偏光显微镜评估病变深度（Ld）、QLF 检测的荧光损失（ΔF）和分光光度计检测的亮度（ΔL）来评估病变严重程度。分析 Ld、ΔF 和 ΔL 之间的关系。
Ld 随着脱矿时间的延长而显著变深（p < 0.05）。ΔF 随着脱矿时间的延长而显著降低（p < 0.05），ΔL 随着脱矿时间的延长而显著增加（p < 0.05）。较浅的病变（Ld≤200μm），ΔF 与 Ld 之间具有较强的相关性（r = -0.898，p < 0.001），而在较深的病变（Ld>200μm）中，ΔL 与 Ld 之间具有较强的相关性（r = 0.858，p < 0.001）。研究结果表明，QLF 系统更适用于浅层早期龋的检测，而分光光度计更适用于深层病变的检测。
