Aquatic Animal Health and Diseases Department, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Fish Shellfish Immunol. 2018 Sep;80:319-324. doi: 10.1016/j.fsi.2018.06.032. Epub 2018 Jun 18.
The present study investigated the possible effects of different anesthetic agents including MS222 (50 ppm), 2-Phenoxyethanol (2-PE) (0.2 mL L) and clove oil (25 ppm), on cutaneous mucosal immune parameters in rainbow trout (Oncorhynchus mykiss). The induction and recovery times for each anesthetic agent were assessed. Also, the immune parameters were measured in skin mucus, 1 and 24 h post anesthesia. No significant difference was observed among treatments at 1 h post-anesthesia except for bactericidal and alkaline phosphatase (ALP) activities which was significantly enhanced in fish exposed to 2-PE compared to other anesthetics. At 24 h post-anesthesia, most of the skin mucosal immune parameters were increased upon exposure to clove oil but decreased following exposure to 2-PE. However, no significant change was noticed after MS222 exposure. These results demonstrated that the anesthetics type should be considered to avoid possible immunosuppression in farmed fish. Furthermore, the present results could be useful for better understanding of alterations in cutaneous mucosal immunity in response to chemical stressors such as anesthetic agents.
本研究调查了不同麻醉剂（包括 50ppm 的 MS222、0.2ml/L 的 2-苯氧乙醇和 25ppm 的丁香油）对彩虹鳜皮肤黏膜免疫参数的可能影响。评估了每种麻醉剂的诱导和恢复时间。此外，在麻醉后 1 和 24 小时测量了皮肤黏液中的免疫参数。除了杀菌和碱性磷酸酶（ALP）活性外，在麻醉后 1 小时，各处理组之间没有观察到显著差异，与其他麻醉剂相比，暴露于 2-PE 的鱼的杀菌和 ALP 活性显著增强。在麻醉后 24 小时，与暴露于其他麻醉剂的鱼相比，暴露于丁香油的鱼的大多数皮肤黏膜免疫参数增加，但暴露于 2-PE 的鱼的皮肤黏膜免疫参数减少。然而，暴露于 MS222 后没有观察到显著变化。这些结果表明，为了避免养殖鱼类可能的免疫抑制，应考虑麻醉剂的类型。此外，本研究结果有助于更好地理解皮肤黏膜免疫对化学应激因子（如麻醉剂）的反应的变化。
