Resta S, Luby J P, Rosenfeld C R, Siegel J D
Science. 1985 Sep 6;229(4717):978-81. doi: 10.1126/science.2992091.
Coronavirus-like particles were found by electron microscopy in stools from infants with necrotizing enterocolitis. Stool samples from these infants as well as control specimens were passaged in cultures of human fetal intestinal organs. Two samples yielded virus-like particles and these have now been passaged 14 times (HEC 14). Gradient-purified HEC 14 strains had typical coronavirus morphology on electron microscopy and contained five major proteins with molecular sizes ranging from 190 to 23 kilodaltons. Infants with necrotizing enterocolitis developed specific antibody to the viral antigens between the acute and convalescent stages of the disease, as shown by examining serum specimens by single radial hemolysis, immunoenzymatic assay, and Western immunoblotting. No cross-reactivity was shown with other coronavirus strains tested, or with the newly isolated viruses of the Breda-Berne group, responsible for calf or horse diarrhea.
通过电子显微镜在坏死性小肠结肠炎婴儿的粪便中发现了冠状病毒样颗粒。将这些婴儿的粪便样本以及对照样本接种到人类胎儿肠道器官培养物中传代。两份样本产生了病毒样颗粒，目前已传代14次（HEC 14）。梯度纯化的HEC 14毒株在电子显微镜下具有典型的冠状病毒形态，包含5种主要蛋白质，分子大小在190至23千道尔顿之间。通过单放射溶血、免疫酶测定和Western免疫印迹检测血清样本发现，坏死性小肠结肠炎婴儿在疾病急性期和恢复期之间产生了针对病毒抗原的特异性抗体。与所检测的其他冠状病毒毒株或与导致小牛或马腹泻的布雷达-伯尔尼组新分离病毒均未显示交叉反应。
