Neurology Department, Beijing Hospital, National Center of Gerontology, No.1 Dahua Road, Dong Dan, Beijing, 100730, People's Republic of China.
The MOH Key Laboratory of Geriatrics, Beijing Hospital, National Center of Gerontology, No.1 Dahua Road, Dong Dan, Beijing, 100730, People's Republic of China.
Lipids Health Dis. 2018 Jun 19;17(1):143. doi: 10.1186/s12944-018-0795-4.
The mortality of atherosclerotic cerebrovascular disease is on the rise, and changes in intimal and media thickness are a leading cause of cerebral ischemia-related death. Levels of low density lipoprotein cholesterol (LDLC), total cholesterol (TC), and chronic stress (CS) are all recognized risk factors for atherosclerosis (AS). However, the leading independent risk factor is indistinct. This study explored the effects of chronic stress, LDLC, and TC on AS and intimal and media thickness, preliminarily explored the main risk factor of AS, and analyzed the related histocyte mechanisms for macrophages and endothelial cells.
Conditions include normal, high-fat diet (HF), and HF plus CS. The correlations between intimal and media thickness and general risk factors were analyzed using χ2, Spearman's rho test, and multiple linear regression. Univariate Cox regression was used to identify potential factors that affect the non-depression time (NDT). We performed a ROC curve to determine the ability of this condition to predict the thickness. Immunohistochemistry was implemented to detect macrophagocytes and endotheliocytes.
Based on χ2 and Spearman's rho test, LDLC, TC, and CS are all related with intimal and media thickness (P < 0.05). However, in multiple linear regression, CS is still a risk factor of thickness (P < 0.05) but LDLC and TC are not. High levels of LDLC, TC, and CS were correlated with poor NDT (P < 0.05). This condition can predict the thickness sensitively. The endarterium is richest in macrophagocytes, and the arrangement of endotheliocytes is disordered and cracked under CS.
CS is the main independent risk factor for AS and intimal (and media) thickness, rather than LDLC or TC.
动脉粥样硬化性脑血管病的死亡率呈上升趋势，而内膜和中膜厚度的变化是导致与脑缺血相关的死亡的主要原因。低密度脂蛋白胆固醇（LDLC）、总胆固醇（TC）和慢性应激（CS）水平均被认为是动脉粥样硬化（AS）的危险因素。然而，主要的独立危险因素并不明确。本研究探讨了 CS、LDLC 和 TC 对 AS 和内膜及中膜厚度的影响，初步探讨了 AS 的主要危险因素，并分析了巨噬细胞和内皮细胞的相关组织细胞机制。
包括正常、高脂饮食（HF）和 HF 加 CS 三种条件。采用 χ2、Spearman 秩相关检验和多元线性回归分析内膜和中膜厚度与一般危险因素的相关性。采用单因素 Cox 回归分析潜在的影响非抑郁时间（NDT）的因素。绘制 ROC 曲线，以确定该条件预测厚度的能力。采用免疫组织化学方法检测巨噬细胞和内皮细胞。
基于 χ2 和 Spearman 秩相关检验，LDLC、TC 和 CS 均与内膜和中膜厚度相关（P<0.05）。然而，多元线性回归分析显示，CS 仍然是厚度的危险因素（P<0.05），而 LDLC 和 TC 则不是。高水平的 LDLC、TC 和 CS 与较差的 NDT 相关（P<0.05）。该条件对厚度具有较高的预测敏感性。动脉内膜中巨噬细胞最为丰富，CS 下内皮细胞排列紊乱、破裂。
CS 是 AS 和内膜（和中膜）厚度的主要独立危险因素，而不是 LDLC 或 TC。
