Stefanowska Karolina, Woźniak Magdalena, Sip Anna, Mrówczyńska Lucyna, Majka Jerzy, Kozak Wojciech, Dobrucka Renata, Ratajczak Izabela
Department of Chemistry, Faculty of Forestry and Wood Technology, Poznan University of Life Sciences, Wojska Polskiego 75, 60625 Poznań, Poland.
Department of Biotechnology and Food Microbiology, Faculty of Food Science and Nutrition, Poznan University of Life Sciences, Wojska Polskiego 48, 60627 Poznań, Poland.
J Funct Biomater. 2023 Jul 9;14(7):358. doi: 10.3390/jfb14070358.
Chitosan is a natural and biodegradable polymer with promising potential for biomedical applications. This study concerns the production of chitosan-based materials for future use in the medical industry. Bioactive substances-caffeine and ethanolic propolis extract (EEP)-were incorporated into a chitosan matrix to increase the bioactivity of the obtained films and improve their mechanical properties. Acetic and citric acids were used as solvents in the production of the chitosan-based films. The obtained materials were characterized in terms of their antibacterial and antifungal activities, as well as their mechanical properties, including tensile strength and elongation at break. Moreover, the chemical structures and surface morphologies of the films were assessed. The results showed that the solution consisting of chitosan, citric acid, caffeine, and EEP exhibited an excellent antiradical effect. The activity of this solution (99.13%) was comparable to that of the standard antioxidant Trolox (92.82%). In addition, the film obtained from this solution showed good antibacterial activity, mainly against and . The results also revealed that the films produced with citric acid exhibited higher activity levels against pathogenic bacteria than the films obtained with acetic acid. The antimicrobial effect of the chitosan-based films could be further enhanced by adding bioactive additives such as caffeine and propolis extract. The mechanical tests showed that the solvents and additives used affected the mechanical properties of the films obtained. The film produced from chitosan and acetic acid was characterized by the highest tensile strength value (46.95 MPa) while the chitosan-based film with citric acid showed the lowest value (2.28 MPa). The addition of caffeine and propolis to the film based on chitosan with acetic acid decreased its tensile strength while in the case of the chitosan-based film with citric acid, an increase in strength was observed. The obtained results suggested that chitosan films with natural bioactive substances can be a promising alternative to the traditional materials used in the medical industry, for example, as including biodegradable wound dressings or probiotic encapsulation materials.
壳聚糖是一种天然的可生物降解聚合物，在生物医学应用方面具有广阔的潜力。本研究关注用于未来医疗行业的壳聚糖基材料的生产。将生物活性物质——咖啡因和乙醇蜂胶提取物（EEP）——掺入壳聚糖基质中，以提高所得薄膜的生物活性并改善其机械性能。在壳聚糖基薄膜的生产中，使用乙酸和柠檬酸作为溶剂。对所得材料的抗菌和抗真菌活性以及机械性能（包括拉伸强度和断裂伸长率）进行了表征。此外，还评估了薄膜的化学结构和表面形态。结果表明，由壳聚糖、柠檬酸、咖啡因和EEP组成的溶液表现出优异的抗自由基作用。该溶液的活性（99.13%）与标准抗氧化剂Trolox（92.82%）相当。此外，从该溶液中获得的薄膜表现出良好的抗菌活性，主要针对[具体细菌名称缺失]和[具体细菌名称缺失]。结果还表明，用柠檬酸制备的薄膜对病原菌的活性水平高于用乙酸制备的薄膜。通过添加咖啡因和蜂胶提取物等生物活性添加剂，可以进一步增强壳聚糖基薄膜的抗菌效果。机械测试表明，所使用的溶剂和添加剂会影响所得薄膜的机械性能。由壳聚糖和乙酸制成的薄膜的拉伸强度值最高（46.95MPa），而含柠檬酸的壳聚糖基薄膜的值最低（2.28MPa）。向基于壳聚糖和乙酸的薄膜中添加咖啡因和蜂胶会降低其拉伸强度，而在含柠檬酸的壳聚糖基薄膜的情况下，则观察到强度增加。所得结果表明，含有天然生物活性物质的壳聚糖薄膜可能是医疗行业中传统材料的一种有前途的替代品，例如可作为可生物降解的伤口敷料或益生菌封装材料。
