College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, Guangxi, China.
Anal Bioanal Chem. 2018 Aug;410(21):5165-5172. doi: 10.1007/s00216-018-1170-z. Epub 2018 Jun 19.
A new molecularly imprinted polymer electrochemiluminescence (ECL) sensor was developed for the detection of clopyralid (CPD) based on enzyme-biocatalyzed amplification. CdTe quantum dots were immobilized on the surface of an electrode by reaction with p-aminothiophenol preadsorbed on the electrode. Then a molecularly imprinted film was formed by electrochemical polymerization of o-phenylenediamine in the presence of CPD on the CdTe-modified gold electrode. During the analytical cycle, horseradish peroxidase (HRP)-labeled CPD was replaced by CPD in the sample. The amount of HRP on the molecularly imprinted polymer electrode decreased, and then less HO was catalytically decomposed. Subsequently, the ECL intensity of the CdTe-HO system was enhanced. There was a good linear relationship between ECL intensity and the concentration of CPD in the range from 2.0 × 10 to 2.5 × 10 mol/L and in the range from 2.5 × 10 to 3.5 × 10 mol/L. The detection limit was 4.1 × 10 mol/L. The sensor was applied to determine CPD in vegetable samples. Graphical abstract A molecularly imprinted electrochemiluminescence sensor was fabricated for ultratrace clopyralid determination. The sensitivity was significantly improved with the enhancement of the ECL intensity of quantum dot via the enzymatic reaction of HRP.
一种新的分子印迹聚合物电化学发光（ECL）传感器基于酶生物催化放大被开发用于检测氯吡草（CPD）。CdTe 量子点通过与预吸附在电极上的对氨基苯硫酚反应固定在电极表面。然后，在 CdTe 修饰的金电极上，在存在 CPD 的情况下，通过电化学聚合邻苯二胺形成分子印迹膜。在分析循环中，辣根过氧化物酶（HRP）标记的 CPD 被样品中的 CPD 取代。分子印迹聚合物电极上的 HRP 量减少，然后催化分解的 HO 减少。随后，CdTe-HO 体系的 ECL 强度增强。在 2.0×10 到 2.5×10 mol/L 和 2.5×10 到 3.5×10 mol/L 的范围内，ECL 强度与 CPD 浓度之间存在良好的线性关系。检测限为 4.1×10 mol/L。该传感器被用于测定蔬菜样品中的 CPD。
