将微囊化抗菌剂植物杀菌素掺入义齿基托树脂中。

Incorporation of the microencapsulated antimicrobial agent phytoncide into denture base resin.

作者信息

An S, Judge R B, Wong R H, Arzmi M H, Palamara J E, Dashper S G

机构信息

Melbourne Dental School, The University of Melbourne, Carlton, Victoria, Australia.

Dentistry and Oral Health, Griffith University, Southport, Queensland, Australia.

出版信息

Aust Dent J. 2018 Jun 20. doi: 10.1111/adj.12640.

DOI:10.1111/adj.12640

PMID:29923610

Abstract

BACKGROUND

This study aimed to fabricate a denture base resin (DBR) containing phytoncide microcapsules (PTMCs) and determine the mechanical properties of the resin and antifungal activity.

METHODS

Fifty-four heat-cured rectangular DBR specimens (64 × 10 × 3.3 ± 0.2 mm) containing nine concentrations of PTMC between 0 and 5% (wt/wt) were fabricated and subjected to a three-point bending test. A phytoncide release bioassay was developed using DBR containing 0% and 2.5% PTMCs (wt/wt) in a 24 well-plate assay with incubation of Porphyromonas gingivalis at 37 °C for 74 h. The antifungal activity of PTMCs against Candida albicans, in a pH 5.5 acidic environment was determined in a plate assay.

RESULTS

Flexural strength decreased with increasing PTMC concentration from 97.58 ± 4.79 MPa for the DBR alone to 53.66 ± 2.46 MPa for DBR containing 5.0% PTMC. No release of phytoncide from the PTMCs in the DBR was detected at pH 7.4. The PTMCs had a minimal inhibitory concentration of 2.6% (wt/vol) against C. albicans at pH 5.5.

CONCLUSIONS

PTMCs can be added to DBR 2.5% (wt/wt) without adversely affecting flexural strength. PTMCs released the antimicrobial agent at pH 5.5 at concentrations sufficient to inhibit the growth of the C. albicans.

摘要

背景

本研究旨在制备一种含有植物杀菌素微胶囊（PTMCs）的义齿基托树脂（DBR），并测定该树脂的机械性能和抗真菌活性。

方法

制备了54个热固化的矩形DBR标本（64×10×3.3±0.2mm），其中含有9种浓度在0至5%（重量/重量）之间的PTMCs，并进行三点弯曲试验。使用含有0%和2.5% PTMCs（重量/重量）的DBR在24孔板试验中进行植物杀菌素释放生物测定，将牙龈卟啉单胞菌在37°C下孵育74小时。在平板试验中测定了PTMCs在pH 5.5酸性环境中对白色念珠菌的抗真菌活性。