含植物杀菌素微胶囊的三维打印义齿基托的抗真菌效果、表面粗糙度和细胞毒性：一项体外研究。

Antifungal effect, surface roughness, and cytotoxicity of three-dimensionally printed denture base with phytoncide-filled microcapsules: An in-vitro study.

机构信息

Graduate student, Department of Prosthodontics, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea.

Dental Research Institute, Seoul National University School of Dentistry, Seoul, Republic of Korea.

出版信息

J Dent. 2022 May;120:104098. doi: 10.1016/j.jdent.2022.104098. Epub 2022 Mar 20.

DOI:10.1016/j.jdent.2022.104098

PMID:35321828

Abstract

OBJECTIVES

To produce three-dimensionally (3D) printed removable denture bases with antifungal activity using microencapsulation of phytochemicals that inhibit Candida albicans growth.

METHODS

Two types of phytoncide oil extract A and B were micro-encapsulated. The phytoncide-filled microcapsules were mixed with denture base resin for 3D printing with various concentration conditions, and manufactured into the discs by digital light processing. The microcapsule concentrations in 3D-printed discs were 2, 4, 6 and 8wt% for the phytoncide oil A, and 5, 10, 15, 20, and 25wt% for the phytoncide oil B. Nine groups with different microcapsule concentrations and a control group were prepared (n = 5). Microcapsule-containing 3D-printed denture base resin discs were evaluated in terms of surface roughness, polymerization, antifungal activity, and its persistence against C. albicans, and cytotoxicity.

RESULTS

There was no significant difference amongst the surface roughness values of all discs. The polymerization of 3D-printed resin disc with microcapsule was different between phytoncide type A and B. The discs with phytoncide-filled microcapsules at 6wt% for type A and 15wt% for type B showed significant antifungal activities against C. albicans at 4 weeks. All discs were reported to be non-cytotoxic to human gingival fibroblasts.

CONCLUSIONS

Denture base resin discs with antifungal activities were successfully manufactured using phytoncide micro-encapsulation and digital light processing. Considering the antifungal effect and its persistence, surface roughness, polymerization, and cytotoxicity, the optimal microcapsule concentrations for 3D-printed denture bases were 6wt% and 15wt% for phytoncide A and B, respectively.

CLINICAL SIGNIFICANCE

Using micro-encapsulation of phytochemicals such as phytoncide oil, denture base resin materials with antifungal activities can be successfully fabricated by digital light processing.

摘要

目的

使用植物化学物质的微胶囊化来抑制白色念珠菌生长，生产具有抗真菌活性的三维（3D）可移动义齿基托。

方法

微封装了两种类型的植物杀菌油提取物 A 和 B。将充满植物杀菌素的微胶囊与义齿基托树脂混合，以不同的浓度条件进行 3D 打印，并通过数字光处理制成圆盘。3D 打印圆盘的微胶囊浓度分别为植物杀菌油 A 的 2、4、6 和 8wt%，植物杀菌油 B 的 5、10、15、20 和 25wt%。制备了 9 组不同微胶囊浓度的实验组和一组对照组（n=5）。评估了含微胶囊的 3D 打印义齿基托树脂盘的表面粗糙度、聚合、抗真菌活性及其对白色念珠菌的持久性和细胞毒性。