School of Biological and Chemical Sciences, Institute of Bioengineering, and Materials Research Institute , Queen Mary University of London , Mile End Road , London , E1 4NS , United Kingdom.
Department of Chemistry and Chemical Engineering , Chalmers University of Technology , Gothenburg , 412 96 , Sweden.
Nano Lett. 2018 Jul 11;18(7):4130-4135. doi: 10.1021/acs.nanolett.8b00856. Epub 2018 Jun 26.
Here we report on the fabrication of reconfigurable and solution processable nanoscale biosensors with multisensing capability, based on single-walled carbon nanotubes (SWCNTs). Distinct DNA-wrapped (hence water-soluble) CNTs were immobilized from solution onto different prepatterned electrodes on the same chip, via a low-cost dielectrophoresis (DEP) methodology. The CNTs were functionalized with specific, and different, aptamer sequences that were employed as selective recognition elements for biomarkers indicative of stress and neuro-trauma conditions. Multiplexed detection of three different biomarkers was successfully performed, and real-time detection was achieved in serum down to physiologically relevant concentrations of 50 nM, 10 nM, and 500 pM for cortisol, dehydroepiandrosterone-sulfate (DHEAS), and neuropeptide Y (NPY), respectively. Additionally, the fabricated nanoscale devices were shown to be reconfigurable and reusable via a simple cleaning procedure. The general applicability of the strategy presented, and the facile device fabrication from aqueous solution, hold great potential for the development of the next generation of low power consumption portable diagnostic assays for the simultaneous monitoring of different health parameters.
在这里，我们报告了基于单壁碳纳米管 (SWCNT) 的可重构和溶液处理的多传感纳米生物传感器的制造。通过低成本的介电泳 (DEP) 方法，将独特的 DNA 包裹（因此水溶性） CNT 从溶液中固定到同一芯片上的不同预图案电极上。SWCNTs 被功能化，具有特定且不同的适体序列，用作应激和神经创伤条件的生物标志物的选择性识别元件。成功地进行了三种不同生物标志物的多重检测，并在血清中实现了实时检测，检测限分别为皮质醇、脱氢表雄酮硫酸盐 (DHEAS) 和神经肽 Y (NPY) 的 50 nM、10 nM 和 500 pM，达到了生理相关浓度。此外，通过简单的清洁程序证明制造的纳米器件具有可重构性和可重复使用性。所提出的策略的通用性以及从水溶液中进行的简便器件制造，为开发用于同时监测不同健康参数的下一代低功耗便携式诊断分析奠定了良好的基础。
