1Agricultural Bio-pharmaceutical Laboratory, Qingdao Agricultural University, Qingdao 266109, PR China.
2Laboratory for Animal Nutrition and Immune Molecular Biology, College of Life Science, Qingdao Agricultural University, Qingdao 266109, PR China.
J Med Microbiol. 2018 Aug;67(8):1181-1190. doi: 10.1099/jmm.0.000766. Epub 2018 Jun 20.
The microbiota composition of faeces and colonic contents were analysed to investigate the mechaninsm by which fermented soybean meal improves intestinal microbial communities, growth and immunity in weaning piglets.
Microbiota were investigated using16S rRNA gene sequencing and systematical bio-information Operational Taxonomic Units; α-diversity analyses indicated that fermented soybean meal increased bacterial species diversity.
The levels of Actinobacteria and Proteobacteia in faeces, and Firmicutes and Tenericutes in the colon, increased significantly in piglets fed fermented soybean meal (P<0.05). The relative abundance of Clostridium sensu stricto1, Lachnospira and Bacteoides had positive correlations with diarrhoea in the piglets. Lactobacillus, Blautia and Clostridium sensu stricto1 levels were correlated with increases in the average daily feed intake of piglets. Lactobacillus and Lachnospira also had positive relationships with IgM levels, and lymphocytes levels were increased relative to Clostridium sensu stricto1. Lymphocyte numbers also increased with higher levels of Blautia and decreased with Clostridium sensu stricto1. Increased levels of Blautia were also correlated with significant increases in white blood cells.
The significant differences in faecal and colonic bacteria were correlated with enhanced immunity and overall improved health in the weaning piglets.
分析粪便和结肠内容物的微生物组成，以研究发酵豆粕改善断奶仔猪肠道微生物群落、生长和免疫的机制。
采用 16S rRNA 基因测序和系统生物信息操作分类单元分析微生物群；α多样性分析表明，发酵豆粕增加了细菌物种多样性。
与对照组相比，发酵豆粕组仔猪粪便中的放线菌和变形菌以及结肠中的厚壁菌和软壁菌水平显著升高（P<0.05）。仔猪腹泻与梭状芽孢杆菌属 1、lachnospira 和拟杆菌属的相对丰度呈正相关。乳酸杆菌、布劳特氏菌和梭状芽孢杆菌属 1 的水平与仔猪平均日采食量的增加呈正相关。乳酸杆菌和lachnospira 与 IgM 水平呈正相关，淋巴细胞水平相对于梭状芽孢杆菌属 1 增加。布劳特氏菌水平升高与白细胞数量显著增加有关，而梭状芽孢杆菌属 1 水平升高与白细胞数量减少有关。布劳特氏菌水平的升高也与白细胞数量的显著增加有关。
粪便和结肠细菌的显著差异与断奶仔猪免疫增强和整体健康状况改善有关。
