Xie Zhengjun, Hu Luansha, Li Yuan, Geng Shijie, Cheng Saisai, Fu Xiongfeng, Zhao Shoumei, Han Xinyan
Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.
Twins' Group Co. Ltd, No. 799 Torch road, Gaoxin District, Nanchang, 330000, Jiangxi, China.
World J Microbiol Biotechnol. 2017 Nov 16;33(12):213. doi: 10.1007/s11274-017-2374-7.
This study investigated the effects of dietary fresh fermented soybean meal (FSM) on the intestinal microbiota and metabolites, bacterial enzyme activity and intestinal morphology of weaning piglets. A total of 64 weaned piglets were randomly allocated into two treatments. A corn-soybean-based diet was used as the control and other treatment was fed the same basal diet containing 15% fresh FSM. The feeding trial lasted for 21 days. Bacterial community structure and diversity in the cecum and colon were assessed using pyrosequencing-based analysis. The results showed that the phylum level, Firmicutes, Bacteroidetes, Proteobacteria and Tenericutes were dominant in the cecum or colon. Gut Firmicutes increased, while Bacteroidetes and Proteobacteria decreased in the fresh FSM-fed piglets. At the genus level, the relative abundances of butyrate-producing bacteria, Lactobacillus and Prevotella were higher in both cecum and colon of fresh FSM fed piglets. Meanwhile, fresh FSM could promote the development of intestinal morphological and reduce the incidence of diarrhea. The results indicated that fresh FSM might change intestinal function by influencing intestinal microenvironment.
本研究调查了日粮中新鲜发酵豆粕（FSM）对断奶仔猪肠道微生物群和代谢产物、细菌酶活性及肠道形态的影响。总共64头断奶仔猪被随机分为两种处理。以玉米-豆粕型日粮作为对照，另一种处理饲喂含有15%新鲜FSM的相同基础日粮。饲养试验持续21天。使用基于焦磷酸测序的分析方法评估盲肠和结肠中的细菌群落结构和多样性。结果表明，在门水平上，厚壁菌门、拟杆菌门、变形菌门和柔膜菌门在盲肠或结肠中占主导地位。在饲喂新鲜FSM的仔猪中，肠道厚壁菌门增加，而拟杆菌门和变形菌门减少。在属水平上，在饲喂新鲜FSM的仔猪的盲肠和结肠中，产丁酸菌、乳酸杆菌和普雷沃氏菌的相对丰度均较高。同时，新鲜FSM可促进肠道形态发育并降低腹泻发生率。结果表明，新鲜FSM可能通过影响肠道微环境来改变肠道功能。
