Sabaratnam Rugivan, Pedersen Andreas J T, Kristensen Jonas M, Handberg Aase, Wojtaszewski Jørgen F P, Højlund Kurt
Section of Molecular Diabetes & Metabolism, Institute of Clinical Research, Institute of Molecular Medicine, University of Southern Denmark, Odense C, Denmark.
Department of Endocrinology, Odense University Hospital, Odense C, Denmark.
Physiol Rep. 2018 Jun;6(12):e13723. doi: 10.14814/phy2.13723.
Regular exercise plays an important role in the prevention and treatment of type 2 diabetes (T2D). The synthesis and secretion of myokines in response to contraction may contribute to the beneficial metabolic effects of exercise. However, some exercise-induced responses may be attenuated in T2D. Here, we investigated whether the effect of acute exercise on selected myokines are impaired in T2D. Skeletal muscle biopsies and blood samples were obtained from 13 men with T2D and 14 weight-matched, glucose-tolerant men before, immediately after and 3-h after acute exercise (60 min cycling) to examine muscle expression and plasma/serum levels of selected myokines. One-hour of exercise increased muscle expression of IL6, FGF21, ANGPTL4, CHI3L1, CTGF and CYR61, of which FGF21, ANGPTL4 and CHI3L1 increased further 3-h into recovery, whereas expression of IL6, CYR61, and CTGF returned to baseline levels. There was no immediate effect of exercise on IL15 expression, but it decreased 3-h into recovery. Plasma IL-6 increased robustly, whereas circulating levels of FGF21, ANGPTL4, IL-15, and CHI3L1 increased only modestly in response to exercise. All returned toward baseline levels 3-h into recovery except for plasma ANGPTL4, which increased further. No significant differences in these responses to exercise were observed between the groups. Our results demonstrate that muscle expression and circulating levels of selected known and putative myokines were equally regulated by acute exercise in patients with T2D and weight-matched controls. This suggests that the potential beneficial metabolic effects of these myokines are not impaired in patients with T2D.
规律运动在2型糖尿病（T2D）的预防和治疗中发挥着重要作用。运动收缩时肌动蛋白的合成与分泌可能有助于运动产生有益的代谢效应。然而，2型糖尿病患者的一些运动诱导反应可能会减弱。在此，我们研究了急性运动对特定肌动蛋白的影响在2型糖尿病患者中是否受损。在急性运动（60分钟骑行）前、运动结束后即刻以及运动后3小时，从13名2型糖尿病男性患者和14名体重匹配的糖耐量正常男性中获取骨骼肌活检样本和血液样本，以检测特定肌动蛋白的肌肉表达及血浆/血清水平。一小时的运动增加了IL6、FGF21,、ANGPTL4、CHI3L1、CTGF和CYR61的肌肉表达，其中FGF21、ANGPTL4和CHI3L1在恢复3小时后进一步增加，而IL6、CYR61和CTGF的表达恢复到基线水平。运动对IL15表达没有即刻影响，但在恢复3小时后其表达下降。血浆IL - 6显著增加，而FGF21、ANGPTL4、IL - 15和CHI3L1的循环水平仅因运动而适度增加。除血浆ANGPTL4进一步增加外，所有指标在恢复3小时后均恢复至基线水平。两组之间在这些运动反应上未观察到显著差异。我们的结果表明，在2型糖尿病患者和体重匹配的对照组中，急性运动对特定已知和假定肌动蛋白的肌肉表达及循环水平的调节是相同的。这表明这些肌动蛋白潜在的有益代谢效应在2型糖尿病患者中并未受损。
