粗糙脉孢菌中正磷酸调节的环磷酸二酯酶的一种调节蛋白。

A regulatory protein for orthophosphate-regulated cyclic phosphodiesterase in Neurospora crassa.

作者信息

Shinohara Y, Furukawa K, Hasunuma K

出版信息

Biochem Biophys Res Commun. 1985 Aug 15;130(3):1015-9. doi: 10.1016/0006-291x(85)91716-4.

DOI:10.1016/0006-291x(85)91716-4

Abstract

A regulatory protein for orthophosphate-regulated cyclic phosphodiesterase (cPDase) was detected in mycelial extracts of Neurospora crassa. The protein, designated neucrassin, was precipitated by ammonium sulfate between 60 to 100% saturation, and fractionated by gel filtration through a TSK-gel column. The molecular weight was estimated to be 65,000. Neucrassin inhibited the hydrolyzing activity of cPDase for cyclic 3',5'-AMP in the presence of MnCl2 in a noncompetitive manner, whereas it stimulated the activity for cyclic 2',3'-AMP over a wide ranges of pH's, between 2.8 to 6.8.

摘要

在粗糙脉孢菌的菌丝体提取物中检测到一种正磷酸盐调节的环磷酸二酯酶（cPDase）的调节蛋白。该蛋白被命名为新孢菌素，在60%至100%饱和度的硫酸铵中沉淀，并通过TSK凝胶柱进行凝胶过滤分级分离。估计其分子量为65,000。在存在氯化锰的情况下，新孢菌素以非竞争性方式抑制cPDase对环3',5'-AMP的水解活性，而在2.8至6.8的广泛pH范围内，它刺激cPDase对环2',3'-AMP的活性。

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引用本文的文献

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Mutations affecting cyclic phosphodiesterases and adenylate cyclase in Neurospora.影响粗糙脉孢菌中环磷酸二酯酶和腺苷酸环化酶的突变

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J Bacteriol. 1987 Oct;169(10):4790-5. doi: 10.1128/jb.169.10.4790-4795.1987.

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