Department of Chemical Engineering and R&D Center for Membrane Technology , Chung Yuan Christian University , Chungli District, Taoyuan 320 , Taiwan R.O.C.
School of Chemical Engineering and Chemistry, Mapúa Institute of Technology , Mapúa University , Muralla St , Intramuros, Manila , 1002 Metro Manila , Philippines.
Langmuir. 2019 Feb 5;35(5):1727-1739. doi: 10.1021/acs.langmuir.8b00634. Epub 2018 Jul 3.
Poly(ethylene terephtalate) (PET)-based materials face general biofouling issues that we addressed by grafting a copolymer of glycidyl methacrylate and sulfobetaine methacrylate, poly(GMA- r-SBMA). The grafting procedure involved a dip-coating step followed by UV-exposure and led to successful grafting of the copolymer as evidenced by X-ray photoelectron spectroscopy and zeta potential measurements. It did not modify the pore size nor the porosity of the PET membranes. In addition, their surface hydrophilicity was considerably improved, with a water contact angle falling to 30° in less than 20 s and 0° in less than 1 min. The effect of copolymer concentration in the coating bath (dip-coating procedure) and UV exposure time (UV step) were scrutinized during biofouling studies involving several bacteria such as Escherichia coli and Stenotrophomonas maltophilia, but also whole blood and HT1080 fibroblasts cells. The results indicate that if all conditions led to improved biofouling mitigation, due to the efficiency of the zwitterionic copolymer and grafting procedure, a higher concentration (15 mg/mL) and longer UV exposure time (at least 10 min) enhanced the grafting density which reflected on the biofouling results and permitted a better general biofouling control regardless of the nature of the biofoulant (bacteria, blood cells, fibroblasts).
聚对苯二甲酸乙二醇酯（PET）基材料面临普遍的生物污垢问题，我们通过接枝甲基丙烯酰氧基乙基琥珀酸酯和甲基丙烯酰氧乙基磺酸钠的共聚物，即聚（GMA-r-SBMA）来解决这些问题。接枝过程包括浸涂步骤和随后的 UV 曝光，通过 X 射线光电子能谱和 ζ 电位测量证明了共聚物的成功接枝。它没有改变 PET 膜的孔径和孔隙率。此外，它们的表面亲水性得到了显著改善，水接触角在不到 20 秒内降至 30°，在不到 1 分钟内降至 0°。在涉及多种细菌（如大肠杆菌和嗜麦芽寡养单胞菌）以及全血和 HT1080 成纤维细胞的生物污垢研究中，研究了共聚物体积分数在涂覆浴（浸涂步骤）和 UV 暴露时间（UV 步骤）中的影响。结果表明，如果所有条件都能改善生物污垢的缓解，由于两性离子共聚物和接枝过程的效率，更高的浓度（15mg/mL）和更长的 UV 暴露时间（至少 10 分钟）提高了接枝密度，这反映在生物污垢的结果上，并能更好地控制一般的生物污垢，而不管生物污染物（细菌、血细胞、成纤维细胞）的性质如何。
