Basic Forestry and Proteomics Research Center, College of Forestry, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
BMC Plant Biol. 2018 Jun 20;18(1):125. doi: 10.1186/s12870-018-1336-z.
Moso bamboo (Phyllostachys edulis) is a well-known bamboo species of high economic value in the textile industry due to its rapid growth. Phytohormones, which are master regulators of growth and development, serve as important endogenous signals. However, the mechanisms through which phytohormones regulate growth in moso bamboo remain unknown to date.
Here, we reported that exogenous gibberellins (GA) applications resulted in a significantly increased internode length and lignin condensation. Transcriptome sequencing revealed that photosynthesis-related genes were enriched in the GA-repressed gene class, which was consistent with the decrease in leaf chlorophyll concentrations and the lower rate of photosynthesis following GA treatment. Exogenous GA applications on seedlings are relatively easy to perform, thus we used 4-week-old whole seedlings of bamboo for GA- treatment followed by high throughput sequencing. In this study, we identified 932 cis-nature antisense transcripts (cis-NATs), and 22,196 alternative splicing (AS) events in total. Among them, 42 cis-nature antisense transcripts (cis-NATs) and 442 AS events were differentially expressed upon exposure to exogenous GA, suggesting that post-transcriptional regulation might be also involved in the GA response. Targets of differential expression of cis-NATs included genes involved in hormone receptor, photosynthesis and cell wall biogenesis. For example, LAC4 and its corresponding cis-NATs were GA-induced, and may be involved in the accumulation of lignin, thus affecting cell wall composition.
This study provides novel insights illustrating how GA alters post-transcriptional regulation and will shed light on the underlying mechanism of growth modulated by GA in moso bamboo.
毛竹（Phyllostachys edulis）是一种经济价值很高的竹种，由于其生长迅速，在纺织工业中得到了广泛的应用。植物激素作为生长和发育的主要调节因子，是重要的内源性信号。然而，植物激素调节毛竹生长的机制目前尚不清楚。
本研究报道了外源赤霉素（GA）处理显著增加了节间长度和木质素的凝聚。转录组测序结果表明，光合作用相关基因在 GA 抑制基因类中富集，这与 GA 处理后叶片叶绿素浓度降低和光合作用速率下降相一致。外源 GA 处理幼苗相对容易操作，因此我们使用 4 周龄的毛竹幼苗进行 GA 处理，然后进行高通量测序。在这项研究中，我们鉴定了 932 个顺式自然反义转录本（cis-NATs），总共发现了 22196 个可变剪接（AS）事件。其中，42 个 cis-NATs 和 442 个 AS 事件在暴露于外源 GA 时表现出差异表达，表明转录后调控可能也参与了 GA 反应。cis-NATs 的差异表达靶标包括激素受体、光合作用和细胞壁生物发生相关的基因。例如，LAC4 及其对应的 cis-NATs 是 GA 诱导的，可能参与了木质素的积累，从而影响细胞壁的组成。
本研究提供了新的见解，阐明了 GA 如何改变转录后调控，并将为 GA 调节毛竹生长的潜在机制提供启示。
