Bai Yucong, Xie Yali, Cai Miaomiao, Jiang Jutang, Wu Chongyang, Zheng Huifang, Gao Jian
Key Laboratory of National Forestry and Grassland Administration, Beijing for Bamboo & Rattan Science and Technology, International Center for Bamboo and Rattan, Beijing 100102, China.
Plants (Basel). 2023 Aug 1;12(15):2842. doi: 10.3390/plants12152842.
Moso bamboo () is one of the fastest growing plants. Gibberellin (GA) is a key phytohormone regulating growth, but there are few studies on the growth of Moso bamboo regulated by GA. The () gene family was targeted in this study. Chromosomal distribution and collinearity analysis identified 10 genes evenly distributed on chromosomes, and the family genes were relatively conservative in evolution. The genetic relationship of genes had been confirmed to be closest in different genera of plants in a phylogenetic and selective pressure analysis between Moso bamboo and rice. About 1/3 genes experienced positive selective pressure with segmental duplication being the main driver of gene family expansion. Analysis of expression patterns revealed that only six genes were expressed in different organs of shoot development and flowers, that there was redundancy in gene function. Underground organs were not the main site of GA synthesis in Moso bamboo, and floral organs are involved in the GA biosynthesis process. The auxin signaling factor PheARF47 was located upstream of and genes, where PheARF47 regulated through cis-P box elements and cis-AuxRR elements, based on the result that promoter analysis combined with yeast one-hybrid and dual luciferase detection analysis identified. Overall, we identified the evolutionary pattern of genes in Moso bamboo and the possible major synthesis sites of GA, screened for key genes in the crosstalk between auxin and GA, and laid the foundation for further exploration of the synergistic regulation of growth by GA and auxin in Moso bamboo.
毛竹（）是生长最快的植物之一。赤霉素（GA）是调节生长的关键植物激素，但关于GA调节毛竹生长的研究较少。本研究以（）基因家族为研究对象。染色体分布和共线性分析确定了10个基因均匀分布在染色体上，且该家族基因在进化上相对保守。在毛竹与水稻的系统发育和选择压力分析中，已证实基因在不同植物属中的遗传关系最为密切。约1/3的基因经历了正选择压力，片段重复是基因家族扩张的主要驱动力。表达模式分析表明，在笋发育和花的不同器官中仅6个基因表达，基因功能存在冗余。地下器官不是毛竹GA合成的主要部位，花器官参与GA生物合成过程。基于启动子分析结合酵母单杂交和双荧光素酶检测分析的结果，生长素信号因子PheARF47位于和基因的上游，其中PheARF47通过顺式P盒元件和顺式AuxRR元件调节。总体而言，我们确定了毛竹中基因的进化模式以及GA可能的主要合成部位，筛选了生长素与GA相互作用中的关键基因，为进一步探索GA和生长素在毛竹生长中的协同调节奠定了基础。
