痰中肝素结合蛋白作为囊性纤维化患儿肺部炎症、肺功能和细菌负荷的标志物。
Heparin-binding protein in sputum as a marker of pulmonary inflammation, lung function, and bacterial load in children with cystic fibrosis.
机构信息
Department of Clinical Sciences Lund, Division of Infection Medicine, BMC B14, Lund University, Skåne University Hospital, Tornavägen 10, SE-22184, Lund, Sweden.
Skåne University Hospital, Clinic of Paediatrics, Lund, Sweden.
出版信息
BMC Pulm Med. 2018 Jun 20;18(1):104. doi: 10.1186/s12890-018-0668-7.
BACKGROUND
Cystic fibrosis (CF) is associated with bacterial pulmonary infections and neutrophil-dominated inflammation in the airways. The aim of this study was to evaluate the neutrophil-derived protein Heparin-binding protein (HBP) as a potential sputum marker of airway inflammation and bacterial load.
METHODS
Nineteen CF patients, aged 6-18 years, were prospectively followed for 6 months with sputum sampling at every visit to the CF clinic. A total of 41 sputum samples were collected. Sputum-HBP was analysed with ELISA, neutrophil elastase activity with a chromogenic assay, and total bacterial load with RT-PCR of the 16 s rDNA gene. Data were compared to lung function parameters and airway symptoms.
RESULTS
HBP and elastase correlated to a decrease in FEV%predicted compared to the patients´ individual baseline pulmonary function (∆FEV), but not to bacterial load. Area under the receiver operating characteristic curve values for the detection of > 10% decrease in ∆FEV were 0.80 for HBP, 0.78 for elastase, and 0.54 for bacterial load.
CONCLUSIONS
Sputum HBP is a promising marker of airway inflammation and pulmonary function in children with CF.
背景
囊性纤维化（CF）与肺部细菌感染和气道中以中性粒细胞为主的炎症有关。本研究旨在评估中性粒细胞衍生蛋白肝素结合蛋白（HBP）作为气道炎症和细菌负荷的潜在痰标志物。
方法
19 名年龄在 6-18 岁的 CF 患者前瞻性随访 6 个月，每次就诊时采集痰液样本。共采集了 41 份痰标本。用 ELISA 分析痰 HBP，用显色测定法分析中性粒细胞弹性蛋白酶活性，用 16s rDNA 基因的 RT-PCR 分析总细菌负荷。将数据与肺功能参数和气道症状进行比较。
结果
HBP 和弹性蛋白酶与 FEV%predicted 较患者个体基线肺功能（∆FEV）的下降相关，但与细菌负荷无关。用于检测 ∆FEV 下降>10%的受试者工作特征曲线下面积值分别为 HBP 的 0.80、弹性蛋白酶的 0.78 和细菌负荷的 0.54。
结论
痰 HBP 是 CF 儿童气道炎症和肺功能的有前途的标志物。
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