用于生物标志物检测的空间分辨肽- DNA纳米组装体：DNA定向固定与纳米嫁接的协同作用

Spatially Resolved Peptide-DNA Nanoassemblages for Biomarker Detection: A Synergy of DNA-Directed Immobilization and Nanografting.

作者信息

Adedeji Abimbola Feyisara, Ambrosetti Elena, Casalis Loredana, Castronovo Matteo

机构信息

Centro di Coordinamento Regionale per le Malattie rare, Azienda Sanitaria Universitaria Integrata di Udine, Udine, Italy.

School of Food Science and Nutrition, University of Leeds, Leeds, UK.

出版信息

Methods Mol Biol. 2018;1811:151-162. doi: 10.1007/978-1-4939-8582-1_10.

DOI:10.1007/978-1-4939-8582-1_10

PMID:29926451

Abstract

Peptide microarrays are becoming a promising alternative to protein microarrays due to the challenges associated with protein immobilization and purification. Here, we put forward a novel experimental-based approach that combines DNA-directed immobilization, nanografting, and atomic force height measurements to immobilize computationally designed cyclic peptide on an ultra-flat gold substrate. This procedure yields peptide-DNA nanoarrays, which can bind to the solvent-exposed site on the Beta-2-microglobulin (β2m).

摘要

由于蛋白质固定和纯化存在挑战，肽微阵列正成为蛋白质微阵列的一种有前景的替代方法。在此，我们提出一种基于实验的新方法，该方法结合了DNA定向固定、纳米接枝和原子力高度测量，以将通过计算设计的环肽固定在超平的金基底上。此过程产生肽-DNA纳米阵列，其可与β2-微球蛋白（β2m）上暴露于溶剂的位点结合。