Bano Fouzia, Fruk Ljiljana, Sanavio Barbara, Glettenberg Maximilian, Casalis Loredana, Niemeyer Christof M, Scoles Giacinto
ELETTRA, Sincrotrone Trieste, Trieste, 34012, Italy.
Nano Lett. 2009 Jul;9(7):2614-8. doi: 10.1021/nl9008869.
Atomic force microscopy nanografting was utilized to prepare DNA nanopatches of different sizes (200 x 200 to 1000 x 1000 nm(2)) onto which DNA-protein conjugates can be anchored through DNA-directed immobilization. Height measurements were used to assess the binding of the proteins as well as their subsequent interaction with other components, such as antibodies. The results indicate that nanografted patch arrays are well suited for application in biosensing and could enable the fabrication of multifeature protein nanoarrays.
利用原子力显微镜纳米移植技术制备不同尺寸（200×200至1000×1000 nm²）的DNA纳米贴片，通过DNA定向固定可将DNA-蛋白质缀合物锚定在其上。通过高度测量来评估蛋白质的结合情况以及它们随后与其他成分（如抗体）的相互作用。结果表明，纳米移植贴片阵列非常适合用于生物传感，并且能够制造多特征蛋白质纳米阵列。
