Technical and Macromolecular Chemistry, Paderborn University, Warburger Str. 100, 33098, Paderborn, Germany.
Biohybrid Materials, Department of Bioproducts and Biosystems, Aalto University, P. O. Box 16100, 00076, Aalto, Finland.
Chemistry. 2021 Jun 10;27(33):8564-8571. doi: 10.1002/chem.202100784. Epub 2021 May 4.
The surface-assisted hierarchical assembly of DNA origami nanostructures is a promising route to fabricate regular nanoscale lattices. In this work, the scalability of this approach is explored and the formation of a homogeneous polycrystalline DNA origami lattice at the mica-electrolyte interface over a total surface area of 18.75 cm is demonstrated. The topological analysis of more than 50 individual AFM images recorded at random locations over the sample surface showed only minuscule and random variations in the quality and order of the assembled lattice. The analysis of more than 450 fluorescence microscopy images of a quantum dot-decorated DNA origami lattice further revealed a very homogeneous surface coverage over cm areas with only minor boundary effects at the substrate edges. At total DNA costs of € 0.12 per cm , this large-scale nanopatterning technique holds great promise for the fabrication of functional surfaces.
表面辅助的 DNA 折纸纳米结构的分级组装是制备规则纳米晶格的一种很有前途的方法。在这项工作中，我们探索了这种方法的可扩展性，并在云母-电解质界面上展示了在总面积为 18.75 cm 的范围内形成均匀多晶 DNA 折纸晶格。对在样品表面的随机位置记录的 50 多个单个 AFM 图像的拓扑分析表明，组装晶格的质量和有序性仅存在微小且随机的变化。对量子点修饰的 DNA 折纸晶格的 450 多张荧光显微镜图像的分析进一步显示，在 cm 面积上具有非常均匀的表面覆盖率，只有在基底边缘处有轻微的边界效应。总 DNA 成本为每厘米 0.12 欧元，这种大规模的纳米图案化技术在功能表面的制造方面具有很大的应用前景。
