Thongmee Pun, Itharat Arunporn
J Med Assoc Thai. 2016 Jul;99 Suppl 4:S166-71.
The bark of Erythrina variegata Linn.(Ev) is used in Thai traditional medicine for the treatment of many diseases in Thailand and is an ingredient in the Mahanintangthong remedy (antipyretic) and Lomammapruek remedy (analgesic and anti-inflammatory).
To study anti-inflammatory activities of ethanolic extract of E. variegata in vitro.
Bark of E. variegata was extracted with 95% ethanol. In this study, Griess reagent was used to measure the anti-inflammatory activity by inhibitory effects of extract on nitric oxide production activated by lipopolysaccharide in RAW 264.7 cell lines, COX-2 and TNF-α were also tested by using ELISA techniques.
The ethanolic extract of E. variegata showed potent anti-inflammation properties by inhibiting prostaglandins production through enzyme COX-2 and inhibitory activity against lipopolysaccharide induced nitric oxide production in RAW 264.7 cell lines with an IC50 value of 9.27±0.72 and 47.1±0.21 μg/ml, respectively. However it was not effective against TNF-α release.
The ethanolic extracts of E. variegata bark showed higher inhibitory effect on PGE2 as acute inflammation than inhibitory effect on Nitric oxide production and TNF-α release representing chronic inflammation. This study thus supports the use of E. variegata bark for treatment of inflammation-related diseases by Thai traditional medicine.
刺桐（Erythrina variegata Linn.，Ev）树皮在泰国传统医学中用于治疗多种疾病，是Mahainintangthong疗法（退烧药）和Lomammapruek疗法（镇痛药和抗炎药）的成分。
研究刺桐乙醇提取物的体外抗炎活性。
用95%乙醇提取刺桐树皮。在本研究中，使用格里斯试剂通过提取物对RAW 264.7细胞系中脂多糖激活的一氧化氮产生的抑制作用来测量抗炎活性，还使用酶联免疫吸附测定技术检测了COX-2和TNF-α。
刺桐乙醇提取物通过抑制COX-2酶产生前列腺素显示出强大的抗炎特性，并对RAW 264.7细胞系中脂多糖诱导的一氧化氮产生具有抑制活性，IC50值分别为9.27±0.72和47.1±0.21μg/ml。然而，它对TNF-α释放无效。
刺桐树皮乙醇提取物对作为急性炎症的PGE2的抑制作用高于对代表慢性炎症的一氧化氮产生和TNF-α释放的抑制作用。因此，本研究支持泰国传统医学使用刺桐树皮治疗炎症相关疾病。
