Liberman E A, Minina S V, Mjakotina O L, Shklovsky-Kordy N E, Conrad M
Brain Res. 1985 Jul 8;338(1):33-44. doi: 10.1016/0006-8993(85)90245-8.
Depolarization of neuron membrane was shown to occur during cAMP injection by means of both iontophoresis and pressure. Distension of the neuron by means of blowing with large volumes of solution without cAMP can produce reversible responses. The time course can be made similar to that of the cAMP effect and the responses can be repeated many times in the same neuron. The effect of cAMP can be differentiated from the mechanical response by injecting small volumes of concentrated solutions. The delay in this depolarization response to cAMP is only 0.1-0.3 s. The calculated time of diffusion from the electrode tip to the membrane is about 10 times greater. The similarity of cAMP and mechanical responses and the short delay of the cAMP effect suggests that a cAMP effect may be mediated by mechanical signals in the cytoskeleton.
通过离子电渗法和压力注入环磷酸腺苷（cAMP）时，神经元膜会发生去极化。用大量不含cAMP的溶液吹胀神经元可产生可逆反应。其时间进程可与cAMP效应的时间进程相似，且相同神经元中的反应可重复多次。通过注入少量浓缩溶液，可将cAMP的效应与机械反应区分开来。这种对cAMP去极化反应的延迟仅为0.1 - 0.3秒。计算得出从电极尖端扩散到膜的时间约大10倍。cAMP与机械反应的相似性以及cAMP效应的短延迟表明，cAMP效应可能由细胞骨架中的机械信号介导。
