College of Life Sciences, Collaborative Innovation Center of Cell Biology in Universities of Shandong, Institute of Biomedical Sciences, Shandong Provincial Key Laboratory of Animal Resistance Biology, Shandong Normal University, Jinan, China.
Thorac Cancer. 2018 Aug;9(8):904-910. doi: 10.1111/1759-7714.12792. Epub 2018 Jun 21.
Kinesins are widely expressed, microtubule-dependent motors that play vital roles in microtubule-associated cellular activities, such as cell division and intracellular transport. Eg5, also known as kinesin-5 or kinesin spindle protein, is a member of the kinesin family that contributes to the formation and maintenance of the bipolar mitotic spindle during cell division. Small-molecule compounds that inhibit Eg5 activity have been shown to impair spindle assembly, block mitotic progression, and possess anti-cancer activity. Recent studies focusing on the localization and functions of Eg5 in plants have demonstrated that in addition to spindle organization, this motor protein has non-canonical functions, such as chromosome segregation and cytokinesis, that have not been observed in animals. In this review, we discuss the structure, function, and localization of Eg5 in various organisms, highlighting the specific role of this protein in plants. We also propose directions for the future studies of novel Eg5 functions based on the lessons learned from plants.
驱动蛋白是广泛表达的微管依赖性马达，在微管相关的细胞活动中发挥着至关重要的作用，如细胞分裂和细胞内运输。Eg5，也称为驱动蛋白-5 或驱动蛋白纺锤体蛋白，是驱动蛋白家族的一员，有助于在细胞分裂过程中形成和维持双极有丝分裂纺锤体。已经证明，抑制 Eg5 活性的小分子化合物会损害纺锤体的组装、阻止有丝分裂的进展，并具有抗癌活性。最近的研究集中在 Eg5 在植物中的定位和功能上，表明除了纺锤体组织，这种马达蛋白还具有非典型功能，如染色体分离和胞质分裂，这些功能在动物中没有观察到。在这篇综述中，我们讨论了 Eg5 在各种生物体中的结构、功能和定位，强调了该蛋白在植物中的特定作用。我们还根据从植物中获得的经验，为未来研究 Eg5 的新功能提出了方向。
