Wei Yuli, Mao Haiyan, Xu Yunping, Zou Wencai, Fang Jiasong, Blom Jochen
2National Engineering Research Centre for Oceanic Fisheries, Shanghai Ocean University, Shanghai 201306, PR China.
3The Key Laboratory of Sustainable Exploitation of Oceanic Fisheries Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, PR China.
Int J Syst Evol Microbiol. 2018 Aug;68(8):2462-2467. doi: 10.1099/ijsem.0.002785. Epub 2018 Jun 21.
A novel heterotrophic, Gram-stain-negative, aerobic, rod-shaped bacterium, designated as strain MT5, was isolated from deep seawater in the Mariana Trench and characterized phylogenetically and phenotypically. Bacterial optimal growth occurred at 28 °C (range, 4-45 °C), pH 5-7 (pH 4-11) and with 3-7 % (w/v) NaCl (0-18 %). Phylogenetic analysis based on 16S rRNA gene sequence showed that strain MT5 was related to members of the genus Pseudomonas and shared the highest sequence identities with Pseudomonas pachastrellae CCUG 46540 (99.6 %), Pseudomonas aestusnigri VGXO14 (98.5 %) and Pseudomonas oceani KX 20 (98.4 %). The 16S rRNA gene sequence identities between strain MT5 and other members of the genus Pseudomonas were below 96.7 %. The digital DNA-DNA hybridization values between strain MT5 and the two type strains, P. pachastrellae and P. aestusnigri, were 38.9±2.5 and 25.8±2.4 %, respectively. The average nucleotide identity values between strain MT5 and the two type strains were 90.3 and 87.0 %, respectively. Strain MT5 and the two type strains shared 94.98 and 86.2 % average amino acid identity, and 30 and 33 Karlin genomic signature, respectively. The sole respiratory menaquinone was Q-9. The major polar lipids were phosphatidylethanolamine, diphosphatidyglycerol and phosphatidylglycerol. The predominant cellular fatty acids of strain MT5 were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) (35.3 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (24.1 %), C16 : 0 (15.9 %) and C12 : 0 (7.2 %). The G+C content of the genomic DNA was 61.2 mol%. The combined genotypic and phenotypic data indicated that strain MT5 represents a novel species of the genus Pseudomonas, for which the name Pseudomonas abyssi sp. nov. is proposed, with the type strain MT5 (=KCTC 62295=MCCC 1K03351).
从马里亚纳海沟的深层海水中分离出一株新型异养、革兰氏染色阴性、需氧、杆状细菌,命名为MT5菌株,并对其进行了系统发育和表型特征分析。该细菌的最佳生长条件为28 °C(范围4 - 45 °C)、pH 5 - 7(pH 4 - 11)以及3 - 7%(w/v)NaCl(0 - 18%)。基于16S rRNA基因序列的系统发育分析表明,MT5菌株与假单胞菌属的成员相关,与太平洋假单胞菌CCUG 46540(99.6%)、黑色假单胞菌VGXO14(98.5%)和海洋假单胞菌KX 20(98.4%)的序列同一性最高。MT5菌株与假单胞菌属其他成员之间的16S rRNA基因序列同一性低于96.7%。MT5菌株与两个模式菌株,即太平洋假单胞菌和黑色假单胞菌的数字DNA - DNA杂交值分别为38.9±2.5%和25.8±2.4%。MT5菌株与这两个模式菌株之间的平均核苷酸同一性值分别为90.3%和87.0%。MT5菌株与这两个模式菌株分别共享94.98%和86.2%的平均氨基酸同一性,以及30和33的卡林基因组特征。唯一的呼吸醌为Q - 9。主要极性脂为磷脂酰乙醇胺、二磷脂酰甘油和磷脂酰甘油。MT5菌株的主要细胞脂肪酸为总和特征8(C18 : 1ω7c和/或C18 : 1ω6c)(35.3%)、总和特征3(C16 : 1ω7c和/或C16 : 1ω6c)(24.1%)、C16 : 0(15.9%)和C12 : 0(7.2%)。基因组DNA的G + C含量为61.2 mol%。综合的基因型和表型数据表明,MT5菌株代表假单胞菌属的一个新物种,为此提出名为深海假单胞菌(Pseudomonas abyssi sp. nov.)的新物种,模式菌株为MT5(=KCTC 62295 = MCCC 1K03351)。