Garry R F, Shackleford G M, Berry L J, Bose H R
Cancer Res. 1985 Oct;45(10):5020-6.
Severe weight loss is associated with many malignant diseases of humans and animals. Avian reticuloendotheliosis viruses (RE viruses) induce runting in experimentally infected chickens. Chickens infected with a replication-competent RE virus, reticuloendotheliosis-associated virus, weighed 30-50% less than control birds at the time of death. Chickens infected with reticuloendotheliosis virus, a replication-defective acute leukemia virus, weighed 30% less than the controls. The runting induced by RE viruses does not occur because of reduced food intake. Activities of phosphoenolpyruvate carboxykinase, a key gluconeogenic enzyme in the liver, were reduced approximately 40 and 50%, respectively, by infection with reticuloendotheliosis-associated virus and reticuloendotheliosis virus. RE virus infection, however, did not affect the hepatic pyruvate carboxylase activity, indicating that inhibition of phosphoenolpyruvate carboxykinase is not due to a general inhibition of all liver enzymes. Birds given injections of UV-inactivated RE viruses or reticuloendotheliosis virus-transformed, non-virus-producing tumor cells also exhibited a reduction in phosphoenolpyruvate carboxykinase activity.
严重体重减轻与人类和动物的许多恶性疾病相关。禽网状内皮组织增殖症病毒（RE病毒）在实验感染的鸡中会引发发育迟缓。感染具有复制能力的RE病毒——网状内皮组织增殖症相关病毒的鸡，在死亡时体重比对照鸡轻30% - 50%。感染网状内皮组织增殖症病毒（一种复制缺陷型急性白血病病毒）的鸡，体重比对照鸡轻30%。RE病毒引发的发育迟缓并非因食物摄入量减少所致。肝脏中关键的糖异生酶磷酸烯醇式丙酮酸羧激酶的活性，分别因感染网状内皮组织增殖症相关病毒和网状内皮组织增殖症病毒而降低了约40%和50%。然而，RE病毒感染并未影响肝脏丙酮酸羧化酶的活性，这表明磷酸烯醇式丙酮酸羧激酶的抑制并非由于对所有肝脏酶的普遍抑制。注射紫外线灭活的RE病毒或经网状内皮组织增殖症病毒转化的、不产生病毒的肿瘤细胞的鸡，其磷酸烯醇式丙酮酸羧激酶活性也降低了。
