Gao Wei, Hua Jing, Jia Zhaoyang, Ding Jiping, Han Zengwei, Dong Yun, Lin Qing, Yao Yuan
Department of Radiation Oncology, 9th People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 201999, P.R. China.
Department of Emergency, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China.
Oncol Lett. 2018 Jun;15(6):9884-9888. doi: 10.3892/ol.2018.8589. Epub 2018 Apr 26.
In the present study, the expression level of microRNA-146a-5p (miR-146a-5p) in breast cancer tissue and cell lines was investigated and its effects on proliferation of breast cancer cells. miR-146a-5p expression was detected by reverse transcription-quantitative polymerase chain reaction in breast cancer tissues, paraneoplastic tissue (collected by The Department of Oncology of Changhai Hospital from January 2014 to June 2015), breast cancer cell line MCF-7 and normal breast epithelial cell line MCF 10A. Bioinformatics analysis was conducted to forecast target genes of miR-146a-5p, which was further verified by fluorescent reporter gene detection. The results demonstrated the expression level of miR-146a-5p in breast cancer tissue was significantly higher, compared with paraneoplastic tissue (P<0.01), and the expression level of miR-146a-5p in MCF-7 cells was significantly higher, compared with MCF 10A cells (P<0.01). Overexpression of miR-146a-5p in MCF-7 cells can promote the proliferation, and low expression miR-146a-5p in MCF-7 can inhibit the proliferation. BRCA1 was further identified as a target gene of miR-146a-5p by bioinformatics analysis and fluorescent reporter gene detection. It was concluded that miR-146a-5p is expressed in breast cancer tissue and breast cancer cell line and may regulate the proliferation of MCF-7 via BRCA1.
在本研究中，检测了微小RNA-146a-5p（miR-146a-5p）在乳腺癌组织和细胞系中的表达水平，并研究了其对乳腺癌细胞增殖的影响。采用逆转录-定量聚合酶链反应检测乳腺癌组织、癌旁组织（由长海医院肿瘤科于2014年1月至2015年6月采集）、乳腺癌细胞系MCF-7和正常乳腺上皮细胞系MCF 10A中miR-146a-5p的表达。进行生物信息学分析以预测miR-146a-5p的靶基因，并通过荧光报告基因检测进一步验证。结果显示，与癌旁组织相比，miR-146a-5p在乳腺癌组织中的表达水平显著更高（P<0.01）；与MCF 10A细胞相比，miR-146a-5p在MCF-7细胞中的表达水平显著更高（P<0.01）。在MCF-7细胞中过表达miR-146a-5p可促进增殖，而在MCF-7细胞中低表达miR-146a-5p则可抑制增殖。通过生物信息学分析和荧光报告基因检测进一步确定BRCA1是miR-146a-5p的靶基因。结论是，miR-146a-5p在乳腺癌组织和乳腺癌细胞系中表达，可能通过BRCA1调节MCF-7的增殖。
