Chen Xin, Wang Anping, Yue Xinxia
Department of Digestive Disease, People's Hospital Xinjiang Autonomous Republic, Urumqi, Xinjiang 830001, P.R. China.
Department of Anesthesia, The Second Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, P.R. China.
Oncol Lett. 2018 Jul;16(1):417-424. doi: 10.3892/ol.2018.8609. Epub 2018 May 2.
microRNAs (miRNAs) are important regulators in the development and progression of human gastric cancer (GC). However, the expression and biological function of miR-449c in GC remains unknown. In the present study, miR-449c was demonstrated to be downregulated in GC tissues and cell lines compared with normal. Decreased miR-449c levels were associated with poor prognosis in GC patients. Overexpression of miR-449c inhibited migration and invasion in SGC-7901 cells, while inhibition of miR-449c enhanced migration and invasion in MGC-803 cells. Furthermore, 6-phosphofructo-2-kinase (PFKFB3) was identified to be a downstream target of miR-449c in GC cells. miR-449c directly regulated the expression of PFKFB3 in GC cells. Overexpression of PFKFB3 abrogated the inhibitory effect of miR-449c on the migration and invasion of GC cells. In conclusion, the present study suggests that miR-449c may be a novel biomarker and potential therapeutic target in GC.
微小RNA（miRNA）是人类胃癌（GC）发生发展过程中的重要调节因子。然而，miR-449c在GC中的表达及生物学功能尚不清楚。在本研究中，与正常组织相比，miR-449c在GC组织和细胞系中表达下调。miR-449c水平降低与GC患者预后不良相关。miR-449c过表达抑制SGC-7901细胞的迁移和侵袭，而抑制miR-449c则增强MGC-803细胞的迁移和侵袭。此外，已确定6-磷酸果糖-2-激酶（PFKFB3）是GC细胞中miR-449c的下游靶点。miR-449c直接调控GC细胞中PFKFB3的表达。PFKFB3过表达消除了miR-449c对GC细胞迁移和侵袭的抑制作用。总之，本研究表明miR-449c可能是GC中的一种新型生物标志物和潜在治疗靶点。
