Ackenheil M, Bondy B, Fröhler M, Vakis A
Encephale. 1985 May-Jun;11(3):95-100.
The simultaneous determination of serum CA and their receptors in blood cells offers a possibility to evaluate disturbances of the DA and NA neuronal systems in man. High affinity binding sites for 3H-yohimbine in platelets, 3H-DHA on granulocytes and 3H-spiperone in lymphocytes from healthy control persons, unmedicated (n = 28), and medicated (n = 8) schizophrenics as well as from an unmedicated psychiatric control (n = 14) were investigated. Furthermore, the actual concentration of the circulating CA was determined with HPLC-ECD. In unmedicated schizophrenics as compared to controls, specific binding of 3H-spiperone to lymphocytes was markedly elevated in capacity and less in affinity as compared to controls. For beta2-receptors a significant decrease was found in capacity with no change in affinity. The changes in alpha2-receptors with a slight decrease in capacity were less distinct. The concentrations of circulating CA ranged from normal values up to a more than 3 fold increase in NA and DA, whereas A concentrations were practically unchanged. No overall change in these data was found in the medicated schizophrenic patients. 3H-spiperone binding was characteristically increased only in schizophrenics, but did not elevate above control data in the non-schizophrenic psychiatric control group. Preliminary experiments of family studies suggest that this model could be valuable as a vulnerability marker.
同时测定血清中的儿茶酚胺（CA）及其在血细胞中的受体，为评估人类多巴胺（DA）和去甲肾上腺素（NA）神经元系统的紊乱提供了一种可能性。研究了来自健康对照者（未用药，n = 28）、用药的精神分裂症患者（n = 8）以及未用药的精神科对照者（n = 14）的血小板中3H-育亨宾、粒细胞上的3H-二氢麦角隐亭和淋巴细胞中3H-螺哌隆的高亲和力结合位点。此外，用高效液相色谱-电化学检测法（HPLC-ECD）测定了循环CA的实际浓度。与对照组相比，未用药的精神分裂症患者中，3H-螺哌隆与淋巴细胞的特异性结合在容量上显著升高，而亲和力则低于对照组。对于β2受体，发现容量显著降低，而亲和力无变化。α2受体容量略有降低，变化不太明显。循环CA的浓度范围从正常值到NA和DA升高超过3倍，而A的浓度实际上没有变化。用药的精神分裂症患者这些数据没有总体变化。3H-螺哌隆结合仅在精神分裂症患者中典型性增加，但在非精神分裂症的精神科对照组中未升高至超过对照数据。家族研究的初步实验表明，该模型作为一种易感性标志物可能具有价值。
