Bondy B, Ackenheil M, Birzle W, Elbers R, Fröhler M
Biol Psychiatry. 1984 Oct;19(10):1377-93.
The simultaneous determination of serum catecholamine (CA) and their receptors in blood cells offers the possibility of evaluating disturbances of the dopamine (DA) and noradrenaline (NA) neuronal systems in man. High-affinity binding sites for 3H-yohimbine in platelets, 3H-DHA in granulocytes, and 3H-spiperone in lymphocytes from healthy control persons, unmedicated (n = 28), and medicated (n = 8) schizophrenics, and from an unmedicated psychiatric control group (n = 14) were investigated. Furthermore, the actual concentration of the circulating CA was determined with HPLC-ECD. In unmedicated schizophrenics, as compared with controls, specific binding of 3H-spiperone to lymphocytes was markedly elevated in capacity and less in affinity. For beta 2 receptors a significant decrease was found in capacity with no change in affinity. The changes in alpha 2 receptors, viz. a slight decrease in capacity, were less distinct. The concentrations of circulating CA ranged from normal values to a more than threefold increase in NA and DA, whereas adrenaline (A) concentrations were nearly unchanged. No overall change in these data was found in the medicated schizophrenic patients. 3H-Spiperone binding was characteristically increased only in schizophrenics, but did not rise above control data in the nonschizophrenic psychiatric control group. Preliminary family studies suggest that this model could be valuable as a vulnerability marker.
同时测定血清儿茶酚胺（CA）及其在血细胞中的受体，为评估人类多巴胺（DA）和去甲肾上腺素（NA）神经元系统的紊乱提供了可能。研究了来自健康对照者（未用药，n = 28）、用药的精神分裂症患者（n = 8）、未用药的精神疾病对照组（n = 14）的血小板中3H-育亨宾、粒细胞中3H-DHA以及淋巴细胞中3H-螺哌隆的高亲和力结合位点。此外，用高效液相色谱-电化学检测法测定循环CA的实际浓度。与对照组相比，未用药的精神分裂症患者中，3H-螺哌隆与淋巴细胞的特异性结合在容量上显著升高，亲和力降低。对于β2受体，发现容量显著降低，亲和力无变化。α2受体的变化，即容量略有降低，不太明显。循环CA的浓度范围从正常值到NA和DA增加三倍以上，而肾上腺素（A）浓度几乎没有变化。用药的精神分裂症患者这些数据未发现总体变化。3H-螺哌隆结合仅在精神分裂症患者中典型性增加，但在非精神分裂症的精神疾病对照组中未超过对照数据。初步的家族研究表明，该模型作为一种易感性标志物可能具有价值。
