Mathey-Prevot B, Baltimore D
EMBO J. 1985 Jul;4(7):1769-74. doi: 10.1002/j.1460-2075.1985.tb03849.x.
Several chimeric murine retroviruses were constructed to test whether the gag sequence of Abelson murine leukemia virus (A-MuLV) could influence the in vitro specificity of two sarcoma-inducing oncogenes: src of Rous sarcoma virus and fps of Fujinami sarcoma virus. Although the src- or fps- containing chimerae could transform fibroblasts, they were unable to mimic the action of A-MuLV in causing lymphoid transformation in vitro. A-MuLV-derived gag sequences could, however, functionally replace the 5' end of src and restore the transformation potential of a 5'-truncated src gene. To investigate this functional similarity, we replaced the gag sequence of an A-MuLV virus with the 5' end of src. This recombinant virus behaved like the A-MuLV virus from which it was derived: it transformed both fibroblasts and lymphoid cells in vitro. Taken together, these results suggest that lymphoid transformation in vitro is a specific property of abl and not of src or fps. Furthermore, it shows that a functional homology exists between the gag sequence of A-MuLV and the 5' end of src.
构建了几种嵌合鼠逆转录病毒，以测试阿贝尔逊鼠白血病病毒（A-MuLV）的gag序列是否会影响两种肉瘤诱导癌基因在体外的特异性：劳斯肉瘤病毒的src基因和藤浪肉瘤病毒的fps基因。虽然含有src或fps的嵌合体能够转化成纤维细胞，但它们无法在体外模拟A-MuLV引起淋巴细胞转化的作用。然而，源自A-MuLV的gag序列能够在功能上替代src的5'端，并恢复5'端截短的src基因的转化潜力。为了研究这种功能相似性，我们用src的5'端替换了A-MuLV病毒的gag序列。这种重组病毒的行为与其来源的A-MuLV病毒相似：它在体外既能转化成纤维细胞，也能转化淋巴细胞。综上所述，这些结果表明体外淋巴细胞转化是abl的特异性特性，而非src或fps的特性。此外，这表明A-MuLV的gag序列与src的5'端之间存在功能同源性。
